The materials used included: RPMI 1640 liquid medium (Sigma-Aldrich Co, Ltd, USA), fetal bovine serum (FBS) (Sigma-Aldrich Co, Ltd, USA), penicillin/streptomycin and L-glutamine (Merck KGaA (Darmstadt, Germany)), propidium iodide (Sigma-Aldrich Co, Ltd, USA), RNase A (Sigma, USA), tetramethylrhodamine ethyl ester perchlorate (TMRE)(Sigma, USA), antibodies against β-actin (1:2000 dilution) (Sigma, USA), anti-phospho-p38 kinase antibodies (1:1000 dilution) (Cell Signaling, USA), DC Protein Assay kit (Bio-Rad, USA), cisplatin (cis-Pt, Sigma-Aldrich Co, Ltd, USA).
Rpmi 1640 liquid medium
Manufactured by Merck Group
Sourced in United States
RPMI 1640 liquid medium is a widely used cell culture medium that provides a balanced salt solution and essential nutrients to support the growth and maintenance of a variety of cell types in laboratory settings. It is a standardized, sterile, and ready-to-use product.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Cisplatin cis pt, by Merck Group (1 mentions)
Tetramethylrhodamine ethyl ester perchlorate tmre, by Merck Group (1 mentions)
Antibodies against β actin, by Merck Group (1 mentions)
Rnase a, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Dc protein assay kit, by Bio-Rad (1 mentions)
Morpholinepropanesulfonic acid, by Merck Group (1 mentions)
2 protocols using rpmi 1640 liquid medium
1
Cell Viability Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
The materials used included: RPMI 1640 liquid medium (Sigma-Aldrich Co, Ltd, USA), fetal bovine serum (FBS) (Sigma-Aldrich Co, Ltd, USA), penicillin/streptomycin and L-glutamine (Merck KGaA (Darmstadt, Germany)), propidium iodide (Sigma-Aldrich Co, Ltd, USA), RNase A (Sigma, USA), tetramethylrhodamine ethyl ester perchlorate (TMRE)(Sigma, USA), antibodies against β-actin (1:2000 dilution) (Sigma, USA), anti-phospho-p38 kinase antibodies (1:1000 dilution) (Cell Signaling, USA), DC Protein Assay kit (Bio-Rad, USA), cisplatin (cis-Pt, Sigma-Aldrich Co, Ltd, USA).
+ Expand
The materials used included: RPMI 1640 liquid medium (Sigma-Aldrich Co, Ltd, USA), fetal bovine serum (FBS) (Sigma-Aldrich Co, Ltd, USA), penicillin/streptomycin and L-glutamine (Merck KGaA (Darmstadt, Germany)), propidium iodide (Sigma-Aldrich Co, Ltd, USA), RNase A (Sigma, USA), tetramethylrhodamine ethyl ester perchlorate (TMRE)(Sigma, USA), antibodies against β-actin (1:2000 dilution) (Sigma, USA), anti-phospho-p38 kinase antibodies (1:1000 dilution) (Cell Signaling, USA), DC Protein Assay kit (Bio-Rad, USA), cisplatin (cis-Pt, Sigma-Aldrich Co, Ltd, USA).
2
Preparation of Stock Solutions for Antifungal Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stock solutions of each agent were prepared using dimethyl sulfoxide (DMSO). Stock solutions of 1000 μg.mL-1 were separated into aliquots and stored at -70ºC until they were used. RPMI 1640 liquid medium (Sigma) buffered to a pH 7.0 with 0.165 M morpholinepropanesulfonic acid (Sigma) was used to obtain final tested agent concentrations. To demonstrate that DMSO did not affect the growth of the studied strains, fungal colonies were grown in the presence of final (1% vol/vol) DMSO concentration and compared to growth in DMSO-naïve conditions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!