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E950 digital camera

Manufactured by Nikon

The Nikon E950 is a digital camera designed for laboratory and scientific applications. It features a 3.34-megapixel CCD sensor and can capture images with a resolution of up to 2048 x 1536 pixels. The camera is capable of operating in a variety of modes, including manual, aperture-priority, and shutter-priority. It offers connectivity options such as USB and video output for easy integration with other equipment.

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2 protocols using e950 digital camera

1

Quantitative Stroke Infarct Analysis

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Infarct volume estimation was performed on animals terminated on day 5 post stroke using our previously described procedures [59 (link)]. Briefly, brain slices (2 mm thick) between − 2.00 and + 4.00 mm from bregma were incubated in a 2% TTC solution at 37 °C for 20 min and then photographed using a Nikon E950 digital camera attached to a dissecting microscope. Digitized images were coded and analyzed by an investigator blind to the code. Infarct volume was determined using the Quantity One software package (Bio-Rad, CA) or ImageJ (NIH). Hemorrhagic loci were also visualized in TTC-stained sections by an investigator blind to the code.
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2

Infarct Volume Estimation in Stroke

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Infarct volume estimation was performed on animals terminated on day 5 post stroke using our previously described procedures (Selvamani and Sohrabji, 2010b (link)). Briefly, brain slices (2 mm thick) between −2.00 mm and +4.00 mm from Bregma were incubated in a 2% TTC solution at 37°C for 20 min and later photographed using a Nikon E950 digital camera attached to a dissecting microscope. Infarct volume was determined from digitized images using the Quantity One software package (Bio-Rad CA). The volume of the infarct was expressed as a percentage of the contralateral (non-occluded) hemisphere. To ensure reliable, consistent and unbiased estimation of the infarct zone, all images were first coded and all volumetric traces were performed by an investigator blind to the codes (AS).
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