Fasting venous blood samples were collected in EDTA tubes and centrifuged immediately for 15 min at 4 °C at 1,500 g. The plasma samples were then kept at −80 °C until further analysis. Biochemical parameters such as glucose, triglycerides, total cholesterol, LDL cholesterol (calculated by the Friedewald formula), and HDL cholesterol were measured using colorimetric and enzymatic assays (Spinreact, SA, Spain; Wako Chemicals GmbH, Germany; Polymedco, NY, US; CV < 4%) that were adapted to a Cobas Mira Plus Autoanalyzer (Roche Diagnostics, Spain). The standard lipid profile was analyzed according to Spintrol “H” CAL (Spinreact, SA, Spain) GC–MS reference methods. Spintrol “H” Normal (Spinreact, SA, Spain) was used as a quality control.
Spintrol h cal
Manufactured by Spinreact
Sourced in Spain
The Spintrol "H" CAL is a laboratory instrument designed for the calibration of hematology analyzers. It provides a standardized solution for verifying the accuracy and precision of hematology measurement systems.
Automatically generated - may contain errors
2 protocols using spintrol h cal
1
Lipid Profile Analysis Protocol
2
Comprehensive Lipoprotein Profile Analysis
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Biochemical parameters, lipids, and apolipoproteins were measured using colorimetric, enzymatic and immunoturbidimetric assays (Spinreact, SA, Spain; Wako Chemicals GmbH, Germany; Polymedco, NY, US; CV < 4 %) that were adapted to a Cobas Mira Plus Autoanalyser (Roche Diagnostics, Spain). The lipid profile was analysed according to Spintrol “H” CAL (Spinreact, SA, Spain) GC–MS reference methods. Spintrol “H” Normal (Spinreact, SA, Spain) was used as a quality control. All samples were subjected to the Liposcale test. This advanced lipoprotein test is based on 2D diffusion-ordered (1)H nuclear magnetic resonance (NMR) spectroscopy. This method adds diffusion coefficients to classical NMR determinations to provide a direct measure of mean particle size and number [23 (link)]. CETP activity was measured using a fluorometric assay (BioVision, CA, USA). Lecithin-cholesterol acyltransferase (LCAT) activity was assessed using a fluorometric assay (Calbiochem, CA, USA). Circulating PCSK9 levels were measured using commercial ELISA kits (R&D Systems, MN, USA).
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