Heart rates were assessed in 6 dpf larvae as an index of overall physiological performance and circulation of the organism, to confirm that the addition of MS-222 or the chemical compounds did not significantly affect larval health. For these experiments, larvae were placed in a 24-well plate (1 per well) with 450 µl of 65 mg/l ASW. MS-222 was manually added into each individual well to obtain final concentrations of 75, 125, or 175 mg/l. To assess heart rate after exposure to triclosan, BaP, Phe, or FL, larvae were first anesthetized with 125 mg/l MS-222. Heart rates were then assessed at 36, 68, and 148 minutes, corresponding to the end of the basal, FCCP, and NaN3 measurement segments on the XFe24 Extracellular Flux Analyzer. Heart rate measurements were completed by visual inspection under a Nikon SMZ-1500 zoom stereomicroscope. For each larva, heart rates were counted once for 15 seconds and multiplied by 4 to get the total number of beats per minute (bpm).
Smz 1500 zoom
Manufactured by Nikon
The Nikon SMZ-1500 is a stereomicroscope designed for a wide range of laboratory applications. It features a zoom ratio of 15:1, providing a magnification range of 0.75x to 11.25x. The SMZ-1500 employs a binocular observation tube and offers a long working distance, making it suitable for various sample types and handling requirements.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using smz 1500 zoom
1
Assessing Zebrafish Larval Physiology
2
Analyzing Aluminum Stress Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze root length, five-day-old seedlings grown on agar (for details see Plant Material) were transferred on agar plates containing 1/8 MS, pH 4 supplemented with 200 μM AlCl3. After 9 day incubation, plates were scanned (Canon CanoScan 8800F) and the root growth was measured using the JMicroVision 1.2.7 software.
To measure pollen tubes length, tobacco pollen was transiently transformed with AtNPC4:YFP by particle bombardment. After 6 h of germination in dark, pollen tubes were incubated in liquid simple sucrose medium (pH 5) with or without 50 μM AlCl3 for additional 2 h. Mean growth rate of pollen tubes expressing AtNPC4:YFP and vector-only control was evaluated using the fluorescence microscope Olympus BX-51.
To determine survival rate, 7-day-old seedlings grown on agar (for details see Plant Material) were transferred to 6-well plates with liquid 1/8 Hoagland’s (Kocourková et al., 2011 (link)) solution (pH 4) with 100 μM AlCl3 for 22 days. Plates pictures were taken by Nikon SMZ 1500 zoom stereoscopic microscope coupled to a Nikon DS-5M digital camera. The survival rate was calculated as a number of viable true leaves.
To measure pollen tubes length, tobacco pollen was transiently transformed with AtNPC4:YFP by particle bombardment. After 6 h of germination in dark, pollen tubes were incubated in liquid simple sucrose medium (pH 5) with or without 50 μM AlCl3 for additional 2 h. Mean growth rate of pollen tubes expressing AtNPC4:YFP and vector-only control was evaluated using the fluorescence microscope Olympus BX-51.
To determine survival rate, 7-day-old seedlings grown on agar (for details see Plant Material) were transferred to 6-well plates with liquid 1/8 Hoagland’s (Kocourková et al., 2011 (link)) solution (pH 4) with 100 μM AlCl3 for 22 days. Plates pictures were taken by Nikon SMZ 1500 zoom stereoscopic microscope coupled to a Nikon DS-5M digital camera. The survival rate was calculated as a number of viable true leaves.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!