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U plex glucagon elisa

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The U-plex glucagon ELISA is a laboratory equipment product designed for the quantitative measurement of glucagon levels in biological samples. It is an enzyme-linked immunosorbent assay (ELISA) that utilizes specific antibodies to detect and quantify the presence of glucagon in the sample.

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Tc 324b, by Warner Instruments (2 mentions)

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U-PLEX Glucagon

3 protocols using u plex glucagon elisa

1

In situ Glucagon Secretion Measurement

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In situ measurements of glucagon secretion were performed using the perfused mouse pancreas. Briefly, the aorta was ligated above the coeliac artery and below the superior mesenteric artery and then cannulated. The pancreas was perfused with KRB containing glucose and CYN154806 at a speed of 0.24 ml/min using an Ismatec Reglo Digital MS2/12 peristaltic pump. The perfusate was maintained at 37°C using a Warner Instruments temperature control unit TC-32 4B in conjunction with a tube heater (Warner Instruments P/N 64-0102) and a Harvard Apparatus heated rodent operating table. The effluent was collected in intervals of 1 min. Samples were subsequently stored at -80°C. Glucagon content in perfusate were measured using U-plex glucagon ELISA (Meso Scale Discovery), according to the manufacturer’s protocol.
Vergari E., Denwood G., Salehi A., Zhang Q., Adam J., Alrifaiy A., Wernstedt Asterholm I., Benrick A., Chibalina M.V., Eliasson L., Guida C., Hill T.G., Hamilton A., Ramracheya R., Reimann F., Rorsman N.J., Spilliotis I., Tarasov A.I., Walker J.N., Rorsman P, & Briant L.J. (2020). Somatostatin secretion by Na+-dependent Ca2+-induced Ca2+ release in pancreatic delta-cells. Nature metabolism, 2(1), 32-40.
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2

In situ Glucagon Secretion Measurement

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In situ measurements of glucagon secretion were performed using the perfused mouse pancreas. Briefly, the aorta was ligated above the coeliac artery and below the superior mesenteric artery and then cannulated. The pancreas was perfused with KRB containing glucose and CYN154806 at a speed of 0.24 ml/min using an Ismatec Reglo Digital MS2/12 peristaltic pump. The perfusate was maintained at 37°C using a Warner Instruments temperature control unit TC-32 4B in conjunction with a tube heater (Warner Instruments P/N 64-0102) and a Harvard Apparatus heated rodent operating table. The effluent was collected in intervals of 1 min. Samples were subsequently stored at -80°C. Glucagon content in perfusate were measured using U-plex glucagon ELISA (Meso Scale Discovery), according to the manufacturer’s protocol.
Vergari E., Denwood G., Salehi A., Zhang Q., Adam J., Alrifaiy A., Wernstedt Asterholm I., Benrick A., Chibalina M.V., Eliasson L., Guida C., Hill T.G., Hamilton A., Ramracheya R., Reimann F., Rorsman N.J., Spilliotis I., Tarasov A.I., Walker J.N., Rorsman P, & Briant L.J. (2020). Somatostatin secretion by Na+-dependent Ca2+-induced Ca2+ release in pancreatic delta-cells. Nature metabolism, 2(1), 32-40.
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3

Measuring Islet Hormone Secretion

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Plasma insulin and glucagon were determined using insulin and glucagon mouse sandwich ELISA (10-1113-01 and 10-1281-01 from Mercodia, Sweden). Insulin and glucagon concentrations from ex vivo islet experiments were measured using mouse/rat insulin-glucagon sandwich ELISA (K15145C, Mesoscale Discovery, USA), and somatostatin concentration was determined using radioimmunoassay (Life Science AB, Sweden). Glucagon concentrations from the perfusate of the in situ perfused mouse pancreas were measured using the U-plex Glucagon ELISA (K1515YK, Mesoscale Discovery). All measurements were conducted according to the manufacturers’ protocols.
Kellard J.A., Rorsman N.J., Hill T.G., Armour S.L., van de Bunt M., Rorsman P., Knudsen J.G, & Briant L.J. (2020). Reduced somatostatin signalling leads to hypersecretion of glucagon in mice fed a high-fat diet. Molecular Metabolism, 40, 101021.
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