Hydrochloric acid 2 m hcl

For the detection of proliferating cells, differentiation medium containing 5 µM of 5′-bromo-2′-deoxyuridine (BrdU) (Sigma, Germany) was used for 2 h in all the experimental groups. Later, cell cultures were washed with PBS, fixed in 4% paraformaldehyde (PFA; Sigma) for 10 min, washed with PBS again and treated with hydrochloric acid 2 M (HCl; Merck, Darmstadt, Germany) for 30 min at 37 °C. BrdU is a thymidine analog and can be incorporated into newly synthesized DNA strands of mitotic cells. This incorporation of BrdU into cellular DNA can then be detected by immunocytochemistry using anti-BrdU antibody, allowing an assessment of the cell proliferative rate [49 (link)].

To detect proliferating cells, differentiation medium containing 5 µM of 5′-bromo-2′-deoxyuridine (BrdU) (Sigma) was added to the different experimental groups for 2 h. Cell cultures were then immediately washed with PBS and fixed in 4% paraformaldehyde (PFA; Sigma) for 10 min, then washed with PBS again and treated with hydrochloric acid 2 M (HCl; Merck) for 30 min at 37 °C, and finally revealed by immunocytochemistry. BrdU is a thymidine analog and can be incorporated into newly synthesized DNA strands of mitotic cells. The incorporation of BrdU into cellular DNA can then be detected using anti BrdU antibody, allowing assessment of the cell proliferation rate [44 (link)].

Triblock copolymer Pluronic P-123 (Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)) average M_n ~5800 (Sigma-Aldrich, Steinheim, Germany), (2-Cyanoethyl)triethoxysilane 97% (CTES) (Alfa Aesar, Karlsruhe, Germany), tetraethoxysilane > 99% (TEOS), ferric chloride hexahydrate > 99% (FeCl₃·6H₂O), ferrous chloride tetrahydrate > 98% (FeCl₂·4H₂O), sulfuric acid 95–97% (H₂SO₄), hydrochloric acid 2 M (HCl) (Merck, Darmstadt, Germany), ammonia solution (NH₄OH 25 wt.%) (Chimreactiv, Bucharest, Romania), Methylene Blue Reag. Ph Eur (C₁₆H₁₈ClN₃S·3H₂O), and Malachite Green G analytical standard (C₂₇H₃₄N₂O₄S) (Sigma-Aldrich, Steinheim, Germany) were commercial reagent grade.