Thirteen participants had CRP levels of 10 mg/L or higher. Because removal of those with “high CRP” (i.e., greater than 10) is still debated in the literature (Mac Giollabhui et al., 2020 (link)), we conducted analyses on CRP by 1) retaining those with high CRP and correcting for skewness by taking the natural log (raw CRP skew including “high CRP participants” = 2.81; n_log CRP skew including “high CRP participants” = −0.18), and 2) removing those participants with CRP greater than 10 (raw CRP skew without “high CRP participants” = 1.14).
Tnf α and il 6
TNF-α and IL-6 are cytokine proteins that play key roles in the immune response. TNF-α is involved in systemic inflammation, while IL-6 is a pro-inflammatory cytokine. These proteins can be measured in the lab to assess various physiological and pathological conditions.
Lab products found in correlation
18 protocols using tnf α and il 6
Inflammatory Biomarker Quantification and Preprocessing
ELISA Quantification of Inflammatory Cytokines
Plasma Markers of ECC-Induced Inflammation
To measure ECC-induced organ damage in our model, the right lung and right kidney were harvested, homogenized, and centrifuged as described [12 (link)]. Briefly, tissues were harvested on ice immediately after euthanasia and weighed. An aliquot of tissue (50 mg) was cut into 1 mm3 pieces, added to 500 μL normal saline, and homogenized. Samples were then left standing on ice for 5 min and centrifuged at 3500 ×g for 20 min at 40°C. Supernatants were transferred to Eppendorf tubes and stored at −70°C until analysis. Samples were assayed for TNF-α, IL-6, and NE as for plasma samples.
In Vivo Cytokine Release Analysis
In-Vitro Anti-Inflammatory Assay
Cytokine Profiling in Brain Tissue
Cytokine Release Assay for Immune Cells
Macrophage Cytokine Release Assay
Modulation of Cytokine Responses in IAV Infection
Cytokine Profiling in ARDS Model
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!