C57BL/6 (H-2b), TNFR1−/− (B6.129-Tnfrsf1atm1Mak/J) and TNFR2−/− (B6.129S2-Tn frsf1btm1Mwm/J) mice were purchased from the Jackson Laboratory. Mice were housed in specific pathogen-free conditions at the First Hospital Animal Center of Jilin University. Mice at 6–8 weeks of age were used in experiments. All animal experiments were approved by the Animal Ethical Committee of First Hospital of Jilin University.
B16 and B16-OVA melanoma cells were purchased from ATCC (Rockville, MD). Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), 100 U/mL penicillin and 100 mg/mL streptomycin (both from Invitrogen).
B16 and B16-OVA melanoma cells were purchased from ATCC (Rockville, MD). Cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone), 100 U/mL penicillin and 100 mg/mL streptomycin (both from Invitrogen).