Anti-LDHA antibody (21799-1-AP), anti-TPI1 antibody (10713-1-AP), anti-ARPP19 antibody (11678-1-AP), anti-KIF2A antibody (13105-1-AP) and anti-beta actin antibody (66009-1-Ig) were purchased from Proteintech (Wuhan, China). Total proteins were extracted utilizing SDS (Beyotime, Shanghai, China). The detailed protocols for western blotting were based on previously described methods (22 (link)). A ChemiDoc MP Imaging System (Bio-Rad) was used to detect and normalize the protein expression levels according to the housekeeping gene β‐actin. The protein concentration was measured by the BCA protein assay (Cat. 23225, Thermo Fisher Scientific, Waltham, MA, USA).
Anti beta actin antibody 66009 1 ig
Manufactured by Proteintech
Sourced in China
The Anti-beta actin antibody (66009-1-Ig) is a primary antibody that specifically recognizes and binds to the beta-actin protein. Beta-actin is a ubiquitous cytoskeletal protein found in all eukaryotic cells and is involved in various cellular processes such as cell motility, structure, and signaling.
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2 protocols using anti beta actin antibody 66009 1 ig
1
Western Blot Analysis of Protein Targets
2
Anti-Inflammatory Effects of Urokinase
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Urokinase (UK) for injection (250,000 U; Guangdong Tianpu Biochemical Pharmaceutical Co., Ltd) was obtained from Central Pharmacy of the First Affiliated Hospital of Guangxi Medical University. Sodium pentobarbital used in anesthesia was purchased from Beijing Solarbio Science & Technology Co., Ltd. Antibodies against p65 along with horseradish peroxidase‐conjugated goat anti‐rabbit antibodies and bicinchoninic acid (BCA) assay were purchased from Shanghai Biyuntian Biotechnology Co., Ltd. Anti‐TNF‐α antibodies and anti‐IL‐1β antibodies were purchased from Beijing Solarbio Technology Co., Ltd.
Anti‐IκB alpha antibody (ab32518) and anti‐phospho‐NF‐κB p65 (S536) antibody (ab86299) were purchased from Abcam. Anti‐NF‐κB p65 antibody (10745‐1‐AP) and anti‐beta‐actin antibody (66009‐1‐Ig) were purchased from Proteintech. Anti‐phospho‐p38 MAPK antibody (AP0526), anti‐p38 MAPK antibody (A4771), and goat anti‐rabbit IgG (AS014) were purchased from ABclonal Technology Co., Ltd. Anti‐phospho‐IκBα (S32) antibody (2859T) was purchased from Cell Signaling Technology. BL‐420F biological signal acquisition and analysis system was purchased from Chengdu Taimeng Software Co., Ltd. The needle of a 5 mL syringe is used for puncture.
Anti‐IκB alpha antibody (ab32518) and anti‐phospho‐NF‐κB p65 (S536) antibody (ab86299) were purchased from Abcam. Anti‐NF‐κB p65 antibody (10745‐1‐AP) and anti‐beta‐actin antibody (66009‐1‐Ig) were purchased from Proteintech. Anti‐phospho‐p38 MAPK antibody (AP0526), anti‐p38 MAPK antibody (A4771), and goat anti‐rabbit IgG (AS014) were purchased from ABclonal Technology Co., Ltd. Anti‐phospho‐IκBα (S32) antibody (2859T) was purchased from Cell Signaling Technology. BL‐420F biological signal acquisition and analysis system was purchased from Chengdu Taimeng Software Co., Ltd. The needle of a 5 mL syringe is used for puncture.
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