Mouse anti igg

Manufactured by Santa Cruz Biotechnology

Mouse anti-IgG is a laboratory reagent used to detect and quantify immunoglobulin G (IgG) in biological samples. It functions as a primary antibody that specifically binds to IgG molecules, enabling their identification and measurement.

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Lab products found in correlation

Mouse monoclonal anti flag m2, by Merck Group (1 mentions) Goat anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Mouse anti flag hrp, by Merck Group (1 mentions) Mouse anti histone h2b, by Abcam (1 mentions) Rabbit anti phospho rpa s4 s8, by Fortis Life Sciences (1 mentions) Rabbit anti rad51, by Abcam (1 mentions) Rabbit anti nbs1, by Novus Biologicals (1 mentions) Rabbit anti mdc1, by Abcam (1 mentions) Mouse anti vinculin, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Horseradish peroxidase-conjugated anti-rabbit and anti-mouse IgGs Rabbit anti-mouse IgG HRP-linked antibody Anti-mouse and anti-rabbit IgGs Peroxidase-conjugated goat anti-mouse IgG Texas red-conjugated anti–mouse IgG Biotinylated goat anti-mouse IgG (sc-2013; Mouse anti-GAPDH IgG Anti-mouse IgG coupled to horseradish peroxidase Mouse anti-rabbit IgG-TR Horseradish peroxidase (HRP)-conjugated goat anti-rabbit or goat anti-mouse IgG

2 protocols using mouse anti igg

Chromatin Immunoprecipitation of EBNA1

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ChIP assays were performed as previously described (Chau and Lieberman, 2004 (link)). Briefly 293T cells were plated and transfected as in the plasmid replication assay with FLAG-EBNA1 WT or the Y518F point mutant. Seven days post transfection, cells were synchronized with aphidicolin and S phase cells were harvested for either native or crosslinked ChIP. TaqMan qPCR performed using primers and probe detecting EBV B95.8 genome coordinates 8401–8521 adjacent to FR was designed by Themo-Fisher. Antibodies used were as follows: mouse anti-IgG (Santa Cruz Biotechnology), Trimethyl-histone H3 (Lys4) (Millipore, cat no. 07–473), anti-flag M2 mouse monoclonal (Sigma Aldrich, cat no. F1804).

Dheekollu J., Wiedmer A., Ayyanathan K., Deakyne J.S., Messick T.E, & Lieberman P.M. (2021). Cell Cycle-Dependent EBNA1-DNA Cross-Linking Promotes Replication Termination at oriP and Viral Episome Maintenance. Cell, 184(3), 643-654.e13.

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Immunoprecipitation and Western Blot Analysis

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Rabbit-anti-ID3 (Cell Signaling, Cat#9837) and for IP of endogenous ID3 we used agarose beads conjugated with mouse-anti-ID3(Santa Cruz, Cat#sc-56712). Mouse-anti-beta-Actin (Santa Cruz, Cat#sc-47778). Mouse-anti-Vinculin (Santa Cruz, Cat#sc-25336). Mouse-anti-phospho-Histone H2A.X (S139) (Merck, Cat#05-636). Rabbit-anti-phospho-Histone H2A.X (S139) (Abcam, Cat# ab2893). Rabbit-anti-RAD51 (Calbiochem, Cat#PC130). Rabbit-anti-RAD51 (Abcam, Cat#ab176458). Rabbit-anti-XRCC4 (GenTex, Cat#GTX109632). Rabbit-anti-phospho-RPA (S4/S8) (Bethyl, Cat#A300-245A). Mouse-anti-Histone H2B (Abcam, Cat#ab52484). Rabbit-anti-DNA-PKsc (Cell Signaling, Cat#4602). Rabbit-anti-CtIP (Abcam, Cat#70163). Goat-anti-MDC1 (Santa Cruz, Cat#sc-27737). Rabbit-anti-MDC1 (Abcam, Cat#ab11169). Rabbit-anti-NBS1 (Novus Biologicals, Cat#NB100-143). Mouse-anti-RAD50 (Abcam, Cat#ab89). Rabbit-anti-RECQL (Abcam, Cat#ab151501). Mouse-anti-RPA32 (Santa Cruz, Cat#sc-53496). Mouse-anti-Flag-HRP (Sigma, Cat#A8592). Mouse-anti-IgG (Santa Cruz, Cat#sc-2025). Mouse-anti-CenpF (BD bioscience, Cat#610768). Goat-anti-mouse IgG-HRP (Cell Signaling, Cat#7076P2). Goat-anti-rabbit IgG-HRP (Cell Signaling, Cat#7074S). Goat-anti-mouse IgG-AlexaFluor 594 (Molecular Probes, Cat#A11005). Goat-anti-rabbit IgG-AlexaFluor 488 (Molecular Probes, Cat#A11008).

Bakr A., Hey J., Sigismondo G., Liu C.S., Sadik A., Goyal A., Cross A., Iyer R.L., Müller P., Trauernicht M., Breuer K., Lutsik P., Opitz C.A., Krijgsveld J., Weichenhan D., Plass C., Popanda O, & Schmezer P. (2021). ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition. Nucleic Acids Research, 49(20), 11666-11689.

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