Imaging of in situ hybridization and X-gal staining was performed with Zeiss upright AxioImagerM2 through a 20× objective with tiling mode using Zen Blue 2.3 software. HE staining was imaged with Zeiss upright AxioImagerM2 or Zeiss slide scannerZ1 through a 20× objective. For immunofluorescence, Zeiss LSM780 inverted confocal microscope was used to acquire images through a 20× objective using the Zen Black 11 (service pack 7) software. Adobe Photoshop CS5.1 was employed to process all images.
Upright axioimagerm2
Manufactured by Zeiss
The Upright AxioImagerM2 is a microscope system designed for advanced imaging applications. It features high-quality optics and a range of configuration options to meet various research and industrial requirements.
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Lab products found in correlation
4 protocols using upright axioimagerm2
1
Multimodal Imaging of Gene Expression
2
Multimodal Imaging Techniques Protocol
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Imaging of in situ hybridization and X-gal staining was performed with Zeiss upright AxioImagerM2 through a 20X objective with tiling mode. HE staining was imaged with Zeiss upright AxioImagerM2 or Zeiss slide scannerZ1 through a 20X objective. For immunofluorescence and EdU labeling, images were acquired with Zeiss LSM780 inverted confocal microscope through a 20X objective. All images were processed with adobe Photoshop CS5.1.
3
Visualizing PrkC Localization in Bacteria
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Strains were grown on LB medium at 37 °C. The gfp-prkC gene fusion and all truncated versions of prkC were expressed from the inducible Pxyl promoter in the presence of 0.5% xylose. The PrkC localization was analyzed by fluorescent microscopy on a Zeiss Upright Axio Imager M2 microscope as described previously3 (link).
4
Imaging Protocols for Fluorescent Microscopy
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For all section imaging, sections were mounted with DAPI Fluoromount-G. Images were acquired as stated in the text and figure legends using the Zeiss LSM780 inverted confocal microscope, the Zeiss Upright AxioImagerM2, and the Zeiss Upright fluorescent Apotom microscope. For whole-mount wound imaging, the Zeiss Discovery V12 fluorescent stereomicroscope was used. To acquire long fields of skin sections, the Zeiss Upright AxioImagerM2 was used through a ×20 objective with tiling and stitching mode. Image processing was performed with Adobe Photoshop CS5.1 (Adobe, San Jose, CA).
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