Manganese acetylacetonate

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Manganese acetylacetonate is a coordination complex compound used as a laboratory reagent. It consists of a manganese(II) ion coordinated to three acetylacetonate ligands. The compound is a crystalline solid that is soluble in organic solvents.

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Manganese(II) acetylacetonate (9 citations)

4 protocols using manganese acetylacetonate

Synthesis of Functional Nanomaterials

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Poly(maleic
anhydride-alt-1-octadecene), Mn 30 000–50 000
g/mol (Aldrich),
Milli-Q water (18.2 MΩ, filtered with filter pore size 0.22
μM) from Millipore, acetonitrile (HPLC grade, J. T. Baker) and
tetrahydrofuran anhydride (Carlo Erba, p.a.), iron oxide hydroxide
(Sigma-Aldrich, #371254), iron acetylacetonate (Sigma-Aldrich, 99%),
manganese acetylacetonate (Sigma-Aldrich, #245763), hexadecanediol
(Sigma-Aldrich, 90%), dodecylamine (Sigma-Aldrich, 98%), lauric acid
(Sigma-Aldrich, 99%), benzyl ether (Sigma-Aldrich, 98%), and octadecene
(Sigma-Aldrich, 90%), YCl₃, YbCl₃, ErCl₃, ammonium fluoride, and sodium oleate, tri-n-octylphosphine oxide (Strem, 99%), tri-n-octylphosphine
(Strem, 97%), n-octadecylphosphonic acid (PolyCarbon
Industries, 99%), cadmium oxide (Sigma-Aldrich, 99.99%), selenium
(Sigma-Aldrich, 99.99%), diethylzinc (Sigma-Aldrich, 1 M in hexane)
and hexamethyldisilathiane (Sigma-Aldrich, synthesis grade), ethanol
(Sigma-Aldrich, 99.8%), methanol (Sigma-Aldrich, 99.9%), toluene (Sigma-Aldrich,
99.5%), and hexane (Sigma-Aldrich, 99%) were used without any further
purification. All reagents for cell cytotoxicity assay were purchased
from Gibco (ThermoFisher Scientific).

Materia M.E., Pernia Leal M., Scotto M., Balakrishnan P.B., Kumar Avugadda S., García-Martín M.L., Cohen B.E., Chan E.M, & Pellegrino T. (2017). Multifunctional Magnetic and Upconverting Nanobeads as Dual Modal Imaging Tools. Bioconjugate Chemistry, 28(11), 2707-2714.

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Synthesis and Characterization of Nanoparticles

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Iron acetylacetonate, manganese acetylacetonate, oleic acid, oleylamine, benzyl ether, hexane, ε-caprolactone, tetrahydrofuran (THF), N-(3-dimethylaminopropyl)-Nese acetylacetonate, N-hydroxysuccinimide, stannous octoate, tetramethylammonium hydroxide (TMAH), heparin, L-glutamine, reactive oxygen species (ROS) assay kit, and sodium pyruvate were all purchased from Sigma-Aldrich Co. (St Louis, MO, USA). PEG with a terminal hydroxyl and carboxylic acid functional groups (COOH-PEG-OH) was purchased from JenKem Technology (Beijing, People’s Republic of China). 1,2-Hexadecanediol was obtained from TCI (Shanghai) Development Co., Ltd (Shanghai, People’s Republic of China). Medium 199, Roswell Park Memorial Institute 1640 medium, fetal bovine serum, penicillin/streptomycin, and nonessential amino acid were purchased from Hyclone, Logan, UT, USA. Count Kit-8 (CCK-8) was obtained from Beyotime Biotechnology Company, Beijing, People’s Republic of China. Endothelial cell growth supplement was purchased from Sciencell, CA, Carlsbad, USA. Six-well plates were obtained from Corning Incorporated, Corning, NY, USA.

Gong M., Yang H., Zhang S., Yang Y., Zhang D., Li Z, & Zou L. (2016). Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles. International Journal of Nanomedicine, 11, 4051-4063.

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Synthesis of Functionalized Magnetic Nanoparticles

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Poly(maleic anhydride-alt-1-octadecene), Mn 30,000–50,000 (Aldrich), Milli-Q
water
(18.2 MΩ, filtered with filter pore size 0.22 μM) from
Millipore, acetonitrile (HPLC grade, J. T. Baker) and tetrahydrofuran
anhydride (Carlo Erba, p.a.), iron oxide hydroxide (Sigma-Aldrich,
#371254), iron acetylacetonate (Sigma-Aldrich, 99%), manganese acetylacetonate
(Sigma-Aldrich, #245763), hexadecanediol (Sigma-Aldrich, 90%), dodecylamine
(Sigma-Aldrich, 98%), lauric acid (Sigma-Aldrich, 99%), benzyl ether
(Sigma-Aldrich, 98%), and octadecene (Sigma-Aldrich, 90%), as well
as 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride (EDC)
(Aldrich, commercial grade), O-(2-aminoethyl)-O′-methyl polyethylene glycol (“aminoPEG750”,
Aldrich), N,N-dimethylethylenediamine
(for “tertiary amine functionalization”, Aldrich), 2-(2-pyridyl)ethylamine
(for “pyridine functionalization”, Aldrich), and 2,2′-(ethylendedioxy)bis(ethylamine)
(referred to in this article as “diamine” Aldrich),
tetramethylammonium hydroxide (TMAOH, Sigma-Aldrich) were used without
further purification. Acetonitrile (ACN, not anhydrous) was purchased
from J. T. Baker and stored in the glovebox.

Bigall N.C., Rodio M., Avugadda S., Leal M.P., Di Corato R., Conteh J.S., Intartaglia R, & Pellegrino T. (2022). Scaling Up Magnetic Nanobead Synthesis with Improved Stability for Biomedical Applications. The Journal of Physical Chemistry. a, 126(51), 9605-9617.

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Synthesis and Characterization of Magnetic Nanoparticles

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RAW264.7 cells were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China). Iron acetylacetonate, manganese acetylacetonate, 1.2-hexadecanediol, oleic acid, oleylamine, benzyl ether, hexyl hydride, and Tetramethylammonium Hydroxide (TMAH) were purchased from Sigma-Aldrich (St Louis, MO, USA). Absolute ethyl alcohol and hydrochloric acid were obtained from Chongqing Chuandong Chemical Co., Ltd. (Chongqing, China). Phosphate-buffered saline (PBS), 4% paraformaldehyde, and Cell Counting Kit-8 (CCK-8) were purchased from Boster (Wuhan, China). Penicillin, streptomycin, and L-glutamine were purchased from Beyotime (Shanghai, China). RNAsimple Total RNA kit was obtained from Tiangen (Beijing, China). PrimeScript™ 1st Strand cDNA synthesis kit, and TB Green™ Premix DimerEraser™ kit were purchased from TAKARA (Dalian, China). Dulbecco’s modified eagle medium high-glucose (DMEM-H) and fetal bovine serum (FBS) were purchased from Hyclone (South Logan, UT, USA). Potassium ferrocyanide and eosin solution were obtained from Solarbio (Beijing, China). Trypsin-EDTA (0.25%) was obtained from Gibco (Gaithersburg, MD, USA). All the reagents were used as received without further treatment.

Zhang L., Xiao S., Kang X., Sun T., Zhou C., Xu Z., Du M., Zhang Y., Wang G., Liu Y., Zhang D, & Gong M. (2021). Metabolic Conversion and Removal of Manganese Ferrite Nanoparticles in RAW264.7 Cells and Induced Alteration of Metal Transporter Gene Expression. International Journal of Nanomedicine, 16, 1709-1724.

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