The rho-associated protein kinase inhibitor Y-27632 (Y) to promote the cell differentiation and epidermal growth factor (EGF) originally used to promote the cell proliferation5 (link),7 (link) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), and a p38 MAP kinase inhibitor SB203580 (SB2) from Cayman Chemical (Ann Arbor, MI) was used to block the cell senescence. SB431543 (SB4), originally thought to block the negative effect of transforming growth factor-β on cultures,5 (link),7 (link) Dulbecco's modified Eagle's medium–high glucose and fetal bovine serum were obtained from Gibco Industries Inc. (Langley, OK), and plastic culture plates were obtained from Corning (Corning, Inc., Corning, NY). Oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenyl-hydrazone (FCCP), rotenone and antimycin, UK5099, Bis-2-(5-phenylacetamido-1.3.4-thiadiazol-2-yl) ethyl sulfide (BPTES), and etomoxir were purchased from Agilent Technologies.
Uk5099
Manufactured by Agilent Technologies
The UK5099 is a laboratory instrument designed for spectroscopic analysis. It is a key component in the analytical workflow, providing essential data for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
Etomoxir, by Agilent Technologies (2 mentions)
Carbonyl cyanide 4 trifluoromethoxy phenylhydrazone fccp, by Agilent Technologies (1 mentions)
Y 27632, by Fujifilm (1 mentions)
Oligomycin, by Agilent Technologies (1 mentions)
Plastic culture plates, by Corning (1 mentions)
Rotenone and antimycin, by Agilent Technologies (1 mentions)
Sb203580 sb2, by Cayman Chemical (1 mentions)
Seahorse xfe96 analyzer, by Agilent Technologies (1 mentions)
Seahorse xf cell mito stress test kit, by Agilent Technologies (1 mentions)
Bptes, by Agilent Technologies (1 mentions)
2 protocols using uk5099
1
Promoting Cell Differentiation and Proliferation
2
Cellular Substrate Oxidation Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cellular substrate oxidation was assessed by measuring the changes in OCR when specifically blocking three of the primary substrates that fuel mitochondria. Etomoxir (103672-100; Agilent Technologies) blocks long-chain fatty acids by inhibiting carnitine palmitoyl transferase 1a; UK5099 (103673-100; Agilent Technologies) blocks pyruvate by inhibiting the mitochondrial pyruvate carrier; and BPTES (103674-100; Agilent Technologies) blocks glutamine by inhibiting glutaminase 1. We performed the assay by following the manufacturer’s instructions and the combined pathway inhibitors with the Seahorse XF Cell Mito Stress Test Kit (103015-100; Agilent Technologies) in a XFe96 Seahorse analyzer (Agilent Technologies), as described above. Data were analyzed with Seahorse Analytics software. Data shown are the most representative of three biological replicates. The OCR values were normalized to 104 cells with the CyQUANT Direct Cell Proliferation Assay.
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