Flowcytomix custom assay kits

Manufactured by Thermo Fisher Scientific

FlowCytomix custom assay kits are a multiplex-based solution for the simultaneous detection and quantification of multiple analytes in a single sample. The kits utilize flow cytometry technology to enable the analysis of multiple biomarkers from a small sample volume.

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Lab products found in correlation

Flowcytomix pro 3, by Thermo Fisher Scientific (2 mentions) Facscalibur flow cytometer, by BD (2 mentions)

2 protocols using flowcytomix custom assay kits

Cytokine and Bone Marker Profiling

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Interleukin-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-22, interferon gamma (IFN-γ), macrophage inflammatory protein (MIP-1α), transforming growth factor-beta (TGF-β), and TNF levels were measured in the serum by FlowCytomix custom assay kits (Bender MedSystems), according to the manufacturer’s instructions. Samples were acquired using a FACSCalibur flow cytometer (BD Biosciences). Raw data of the flow cytometry bead assay were analyzed using the FlowCytomix Pro 3.0 software (Bender MedSystems). Carboxy-terminal type I collagen crosslinks (CTX-I; Sunred Biological Technology), human type I procollagen amino-terminal-propeptide (P1NP; Sunred Biological Technology), OPG, SOST, DKK-1, and soluble RANKL (Biomedica Gruppe) were quantified by ELISA according to the manufacturer’s instructions.

Perpétuo I.P., Caetano-Lopes J., Vieira-Sousa E., Campanilho-Marques R., Ponte C., Khmelinskii N., Canhão H., Ainola M, & Fonseca J.E. (2017). Ankylosing Spondylitis Patients Have Impaired Osteoclast Gene Expression in Circulating Osteoclast Precursors. Frontiers in Medicine, 4, 5.

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Cytokine and Bone Turnover Biomarkers

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IL-1β, IL-6, IL-12(p70), IL-17A, IL-23, monocyte chemotactic protein-1 (MCP-1), transforming growth factor-beta (TGF-β) and TNF levels were measured in the serum by FlowCytomix custom assay kits (Bender MedSystems) according to the manufacturer instructions. Samples were acquired with a FACS Calibur flow cytometer (BD Biosciences). Raw data of the flow cytometry bead assay were analyzed by FlowCytomix Pro 3.0 software (Bender MedSystems). Carboxy-terminal type I collagen crosslinks (CTX-I), human type I procollagen amino-terminal-propeptide (P1NP, Sunred Biological technology), osteoprotegerin (OPG), sclerostin (SOST), dickkopf-related protein (DKK)-1 and soluble RANKL (ampli-sRANKL, Biomedica Grouppe) were quantified by enzyme-linked immunosorbent assay (ELISA) in serum samples according to the manufacturer’s instructions.

Perpétuo I.P., Raposeiro R., Caetano-Lopes J., Vieira-Sousa E., Campanilho-Marques R., Ponte C., Canhão H., Ainola M, & Fonseca J.E. (2015). Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Ankylosing Spondylitis. PLoS ONE, 10(12), e0144655.

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