The colony‐forming potential of hematopoietic stem/progenitor cells was assessed by culture on methylcellulose (H4320, STEMCELL Technologies) supplemented with 1% penicillin/streptomycin (Invitrogen) and cytokines, as described previously.17 BFU‐E, CFU‐Mix, CFU‐GM, CFU‐G, and CFU‐M colonies were assessed on day 14. The lineage differentiation potential of hematopoietic stem/progenitor cells was assayed in IMDM basic medium containing 10% FBS and hematopoietic cytokines (100 ng/ml SCF, 50 ng/ml IL‐6, 20 ng/ml FLT3L, 20 ng/ml IL‐3, 10 ng/ml GM‐CSF, 10 ng/ml G‐CSF, 10 ng/ml TPO, and 4 U/ml EPO). Anti‐human CD45‐APC/Cy7 (BioLegend, Catalog No.: 368516), anti‐human CD71‐FITC (BD Biosciences, Catalog No.: 555536), anti‐human GPA(235a)‐APC (BD Biosciences, Catalog No.: 551336), anti‐human CD66B‐PE (BioLegend, Catalog No.: 305105), and anti‐human CD14‐PE/Cy7 (BD Biosciences, Catalog No.: 557742) were used to detect erythrocytes, granulocytes, and monocytes on day 10 of lineage induction.
Anti human cd14 pe cy7
Manufactured by BD
Anti‐human CD14‐PE/Cy7 is a fluorochrome-conjugated monoclonal antibody that binds to the CD14 surface antigen on human cells. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein that serves as a co-receptor for the detection of lipopolysaccharide (LPS) and other pathogen-associated molecular patterns. The PE/Cy7 fluorochrome combination provides a bright signal for flow cytometric detection and analysis.
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Lab products found in correlation
H4320, by STEMCELL (1 mentions)
Anti human cd71 fitc, by BD (1 mentions)
Anti human cd45 apc cy7, by BioLegend (1 mentions)
Anti human cd66b pe, by BioLegend (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti human cd3 apc, by BioLegend (1 mentions)
Anti human cd19 brilliant violet 510, by BioLegend (1 mentions)
Anti human cd45 alexa fluor 700, by BioLegend (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
2 protocols using anti human cd14 pe cy7
1
Hematopoietic Stem Cell Colony Assay
2
Nasal Microbiopsies for Immune Profiling
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Nasal microbiopsies were obtained from the inferior turbinate of each participant using microcurettes without local analgesia. Each sample was dropped immediately into transport medium and transferred to the laboratory in less than 10 min. For immunophenotyping, nasal cells were dislodged from curettes by gentle pipetting and suspension. Cells were then live-stained with an antibody cocktail containing anti-human CD3 APC, anti-human CD19 Brilliant Violet 510, TCR Gamma Delta FITC and anti-human CD45 Alexa Fluor 700 (all BioLegend), anti-human CD14 PE-Cy7 (BD Biosciences), anti-human CD66b PE (eBiosciences) and MR1 Tetramer Bv421 (NIH Tetramer Core Facility). After fixation, samples were acquired using standard methods on an LSR FORTESSA flow cytometer (BD Biosciences) and analysed using Flowjo v10.5.3 (BD Biosciences).
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