Basic dab detection kit 250 001

A post-mortem examination was performed on patient KEN-334-6, who signed an informed consent form, for the French National Brain Bank Network Donation Program NeuroCEB.
The postmortem delay was 11 h. The right half of the brain, including the hemisphere, brainstem, and cerebellum, was fixed by immersion in 4% formaldehyde (10% formalin). The contralateral hemisphere was frozen at -80°C. Formalin-fixed brain samples from selected regions, including the cerebral cortex, hippocampus, basal ganglia, cerebellum, brainstem, spinal cord, and anterior and posterior roots of the spinal nerves, were embedded in paraffin and cut at a thickness of 3 μm. The spinal cord was sampled at the cervical, thoracic, and lumbar levels. The sections were deparaffinized in graded alcohol solutions and stained with hematoxylin-eosin (HE) and HE combined with Luxol fast blue for myelin. Selected sections were immunostained using a Ventana BenchMark stainer (Roche TM , Tucson, AZ, USA). The biotinylated secondary antibody was included in the detection kit (Ventana Medical Systems Basic DAB Detection Kit 250-001). Diaminobenzidine (DAB) was used as a chromogen. The pretreatment and antibodies used for immunohistochemistry are listed in Supplemental Table S2. Genomic DNA was isolated from cerebellum, according to standard procedures.