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5 protocols using n butanol

1

Antioxidant and Acetylcholinesterase Assays

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Sodium dodecyl sulfate, thiobarbituric acid, glutathione standard, n-butanol, and pyridine were purchased from Himedia-Mumbai, India. Aluminum chloride hexahydrate (AlCl3.6H2O) was obtained from Thomas Baker Pvt. Chemicals, Mumbai, India. Acetylcholinesterase (AChE) kit was purchased from Piramal Healthcare Ltd. Thane, Mumbai, India. All other chemicals and reagents used in the present work were of analytical grade.
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2

Lipophilic Eugenol Solubilization and Quantification

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Eugenol, thiobarbituric acid (TBA) and Evans blue were procured from Sigma Aldrich. Hank’s balanced salt solution (HBSS) was procured by Thermo Fisher Scientific. TEP (1, 1, 3, 3–tetra ethoxy propane), sodium dodecyl sulphate (SDS), glacial acetic acid, n-butanol, pyridine, phosphate buffer, hydrogen peroxide (H2O2) and all the other reagents and solvents were purchased from HiMedia Laboratories Pvt. Ltd., Mumbai. Eugenol is lipophilic and therefore it is insoluble in water. Hence it was suspended in 40% propylene glycol (PG) in phosphate buffer (pH 6.8).
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3

Microbiology Lab Techniques Protocol

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Nutrient Agar, nutrient broth, luria bertani broth, muller hinton agar, glucose, dextrose, sucrose, lactose, mannitol, phenol red, starch agar, Gram's iodine, yeast extract, gelatin, skim milk powder, agar, peptone, tryptone, kovac's reagent, mr-vp media, simmons citrate agar, Nutrient Agar, nutrient broth, safranin, crystal violet, vp reagent (i), vp reagent (ii), oxidase disc, ferrous ammonium sulphate, sodium thiosulphate, potassium dihydrogen phosphate, Bradford's reagent, DNS reagent, hydrogen peroxide, petroleum ether, glacial acetic acid, n-Butanol were procured from Himedia Laboratories Pvt. Ltd. India. Trypsin, bile salts, Taq polymerase, dNTPs, α-Amylase procured from Sigma Aldrich, USA.
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4

Preparation and Characterization of MPTP

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MPTP was procured by Sterling biologicals, Ahmedabad while Hank's balanced salt solution (HBSS) was obtained from Invitrogen (Thermo Fisher Scientific), Bangalore. Eugenol, 5,5-dithio-bis-(2nitrobenzoic acid) (DTNB) (Ellman's Reagent) and thiobarbituric acid (TBA) were purchased from Sigma Aldrich (USA). Sodium dodecyl sulphate (SDS), 1,1,3,3-tetraethoxypropane (TEP), trichloroacetic acid, pyridine, n-butanol, glacial acetic acid and all additional solvents and reagents were acquired by HiMedia Laboratories Pvt. Ltd., Mumbai. Eugenol was suspended in 40% propylene glycol in phosphate buffer with pH 6.8 as it is hydrophobic. MPTP is water-soluble so it was dissolved in sterile saline.
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5

Antibacterial Assessment of Natural Compounds

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Chemicals and reagents: Ethanol (OPC Pharmaceutical Company); n-hexane, chloroform, ethyl acetate, n-butanol (VN-Chemsol, Co. Ltd); egg albumin (HIMEDIA); calcium chloride, sodium oxalate, sodium citrate, sodium chloride, Tris-HCl, yeast extract, peptone, diclofenac sodium, and amoxicillin were purchased from Sigma-Aldrich ® Co. Ltd. Besides, the bacterial strains used in this study were Escherichia coli (E. coli) (ATCC ® 25922), Pseudomonas aeruginosa (P. aeruginosa) (ATCC ® 27853) and Staphylococcus aureus (S. aureus) (ATCC ® 29213).
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