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Cell culture vessels

Manufactured by Corning
Sourced in United States

Cell culture vessels are specialized containers designed for the growth and maintenance of cells in a controlled laboratory environment. These vessels provide a suitable substrate and conditions for the propagation of various cell types, including adherent and suspension cultures. The core function of cell culture vessels is to facilitate the controlled culturing and study of cells.

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3 protocols using cell culture vessels

1

Anticancer Nanoparticle Synthesis and Evaluation

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Methanol, toluene, dichloromethane, ethyl acetate, tetrahydrofuran and petroleum ether were purchased from Concord (Tianjin, China). Methoxyl-poly(ethylene glycol) (mPEG5000) and hydrogen peroxide (H2O2) were purchased from Energy Chemical (Shanghai, China). 4,9-dibromo-6,7-bis(4-hexylphenyl)-[1,2,5]thiadiazolo[3,4-g]quinoxaline (DPTQ6-2Br) was purchased from Alfa (Zhengzhou, China). Cisplatin (purity 99%) was purchased from Shandong Boyuan Pharmaceutical Co., Ltd. (Shandong, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sodium dodecyl sulfate (SDS) were purchased from Beyotime (Shanghai, China). Cell culture vessels were purchased from Corning (Corning, NY, USA). Roswell Park Memorial Institute -1640 (RPMI-1640) medium, fetal bovine serum (FBS), 0.25% trypsin-EDTA, and penicillin/streptomycin (P/S) were purchased from Gibco (Gran Island, NY, USA). 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI) was purchased from Sigma-Aldrich (Shanghai, China). 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA), Annexin V-FITC/Propidium iodide (PI) apoptosis detection kit, Hoechst 33258 and Calcein-AM/PI double stain kit, ATP assay kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Female Balb/c mice and female Balb/c nude mice (4–6 weeks) are used in the animal experiments.
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2

Cytotoxicity Evaluation of Compounds

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N-Octyl isocyanate and hydrogen peroxide (H2O2), dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Aladdin (Shanghai, China). Cisplatin (purity 99%) was bought from Kunming Institute of Precious Metals (Yunnan, China). Human serum albumin (HSA) was purchased from Shang Hai Yuan Ye Bio-Technology Co., Ltd (Shanghai, China). 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI), Alexa Fluor 488 (Alex 488), Cy5.5, and Cy7.5 were purchased from Sigma-Aldrich (Shanghai, China). Calcein AM/PI (acetyl methoxy methyl ester/propidium iodide) cell viability kit, DNA extraction kit and Annexin V-FITC apoptosis detection kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit were purchased from Roche (Basel, Switzerland). RPMI-1640 medium, DMEM, 0.25% trypsin–EDTA, fetal bovine serum (FBS), and penicillin/streptomycin (P/S) were purchased from Gibco (NY, USA). Cell culture vessels were purchased from Corning (NY, USA).
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3

Synthesis and Characterization of Cytotoxic Agents

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Cet was obtained from Merck KGaA (Darmstadt, Germany). CisPt was obtained from Boyuan Chemical Company (Shandong, China). Succinic anhydride (SA), 1-isocyanatooctane (C8-NCO), acetone, hydrogen peroxide, ether, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), EDCI, and NHS were obtained from Aladdin (Shanghai, China). Unless otherwise specified, all chemicals were acquired from commercial suppliers and utilized without additional purification. The cell culture vessels were obtained from Corning Inc. (Corning, NY, USA). Dulbecco's modified Eagle's medium (DMEM) containing 4.5 g of glucose, Roswell Park Memorial Institute-1640 (RPMI-1640), trypsin-ethylenediamine tetraacetic acid (EDTA) (0.25%), penicillin/streptomycin, and fetal bovine serum were procured from Gibco (Grand Island, NY, USA). 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), Alexa Fluor® 488, and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) apoptosis assay kit were acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China). More detailed information about materials and method are shown in the Supplementary data.
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