At post-mortem, blood was collected from the heart and placed in Serum Gel-Z tubes (Sarstedt, Numbrecht, Germany), which were then centrifuged at 2000 rpm for 5 min. Serum was collected and stored at −80 °C until used for cytokine analysis. Quantitative analysis of cytokines was performed using a LEGENDplex Rat Th Cytokine Panel (13-plex; Biolegend, San Diego, CA, USA), following the manufacturer’s instructions. Data were acquired using a FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA) and analysed using Legendplex software (Biolegend).
Serum gel z tubes
Manufactured by Sarstedt
Sourced in Germany
Serum Gel-Z tubes are laboratory equipment used for the collection, separation, and storage of serum samples. The tubes contain a gel barrier that facilitates the separation of serum from the cellular components of blood during centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
Legendplex software, by BioLegend (1 mentions)
Legendplex rat th cytokine panel 13 plex, by BioLegend (1 mentions)
Facscanto 2, by BD (1 mentions)
Bio plex pro mouse cytokine th17 panel a 6 plex kit, by Bio-Rad (1 mentions)
Liquichip 200 workstation, by Qiagen (1 mentions)
Bio plex manager software, by Bio-Rad (1 mentions)
2 protocols using serum gel z tubes
1
Cytokine profiling of post-mortem serum
2
Multiplex Cytokine Analysis in Mouse Serum
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Blood was collected from sacrificed mice via inferior vena cava using a 22 G needle and transferred to serum‐gel Z tubes (Sarstedt, Germany), allowed to clot for 30 min at room temperature and centrifuged at 10 000 g for 5 min. The serum was collected and stored frozen until use. To detect interleukin‐1 beta (Il‐1β), interleukin‐6 (Il‐6), and tumour necrosis factor‐alpha (Tnf‐α) simultaneously, Bio‐Plex Pro™ mouse cytokine Th17 panel A 6‐Plex kit (Bio‐Rad, Germany) was used according to the manufacturer's instructions. Samples were diluted at 1:2, and the fluorescence measurement of the beads was performed with the Qiagen LiquiChip 200 workstation (Hilden, Germany). Cytokine concentrations were calculated using Bio‐Plex Manager software (Bio‐Rad, Hercules, CA, USA).
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