C57BL/6 male mice 8–10 weeks of age were used. Mice were held under specific pathogen‐free conditions and kept with free access to food and water at the animal care facility at Ulm University in compliance with the German Law for Welfare of Laboratory Animals. All animal experiments were approved by the responsible authorities at Ulm University. Human adipose‐derived mesenchymal stem cells were purchased from the American Type Culture Collection (ATCC) (LGC Standards GmbH, Wesel Germany). MSCs were seeded at a density of 3 × 103 cells/cm2 in mesenchymal stem cell basal medium with mesenchymal stem cell growth kit, low serum (ATCC), and cultured at 37°C under 5% CO2. MSCs were harvested with Accutase (PAA Laboratories, Pasching, Austria) at 70–80% confluence. MSCs at passages 3–5 were used for subsequent experiments.
Human peripheral neutrophils were isolated from buffy coats (German Red Cross, Ulm, Germany) using the Human Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer's instructions.
Human peripheral neutrophils were isolated from buffy coats (German Red Cross, Ulm, Germany) using the Human Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer's instructions.