The F12 K medium, penicillin/streptomycin antibiotic solution (100×), fetal bovine serum (FBS), trypsin-EDTA solution (1X), amphotericin B (1,000×), phosphate-buffered saline without calcium and magnesium, CdCl2, 25% glutaraldehyde and crystal violet were purchased from Sigma-Aldrich (St. Louis, MO, USA). The human IL-10 (cat. no. ELH-IL10–001) and human IL-1α (cat. no. ELH-IL1alpha-001) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Ray Biotech, Inc. (Norcross, GA, USA).
Penicillin streptomycin antibiotic solution 100
Manufactured by Merck Group
Sourced in United States
Penicillin/streptomycin antibiotic solution (100×) is a sterile, concentrated liquid mixture of the antibiotics penicillin and streptomycin. It is designed for use in cell culture applications to provide broad-spectrum antimicrobial protection against a variety of bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Merck Group (2 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Cdcl2, by Merck Group (2 mentions)
F12k medium, by Merck Group (2 mentions)
Trypsin edta solution, by Merck Group (2 mentions)
Phosphate buffered saline without calcium and magnesium, by Merck Group (1 mentions)
Rneasy kit, by Qiagen (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Crystal violet, by Avantor (1 mentions)
Phosphate buffer solution (pbs), by Merck Group (1 mentions)
Glutaraldehyde, by Avantor (1 mentions)
3 ivt express kit, by Thermo Fisher Scientific (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Docetaxel, by Merck Group (1 mentions)
3 protocols using penicillin streptomycin antibiotic solution 100
1
Cadmium Cytokine Response Assay
2
Fenugreek Leaf Powder Cytotoxicity Assay
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F12K medium, penicillin-streptomycin antibiotic solution (100×), fetal bovine serum (FBS), 0.25% Trypsin-EDTA solution, phosphate buffer solution (PBS), 0.25% Trypsin-EDTA solution and CdCl2 were obtained from Sigma-Aldrich (St. Louis, MO, USA). The dried fenugreek leaf powder was purchased from a local Indian store (Tallahassee, FL, USA). The 3′IVT Express kit and RG230 PM whole genome microarray analysis kit were purchased from Affymetrix (Thermo Fisher Scientific, Inc., Santa Clara, CA, USA). The RNeasy kit was purchased from Qiagen, Inc. (Germantown, MD, USA). Crystal violet, 25% glutaraldehyde, sodium monophosphate and 95% ethanol were purchased from VWR International (Suwanee, GA, USA).
3
Cytotoxicity and ROS Evaluation of Compound
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Chemicals. F12K (A549 and PC-3) media, penicillin-streptomycin anti-biotic solution (100×), fetal bovine serum (FBS), trypsin-EDTA solution (1×), phosphate buffered saline (PBS), 50% glutaraldehyde, crystal violet, IGEPAL CA-630, propidium iodide, 2',7'-Dichlorofluorescin diacetate (DCFDA), Tetramethyl Rhodamine Methyl Ester (Rhodamine 123), Acridine orange (AO), Ethidium bromide (EB), Docetaxel, N-acetylcysteine (NAC) and RNase were obtained from Sigma Aldrich (St. Louis, MO, USA).
Cell culture and cell viability assay. Human cell lines (A549 and PC-3) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured as per the guidelines supplied using the crystal violet dye uptake assay according to our previously reported method (19) (link)(20) (link)(21) (link). Cell viability assay was performed in F12K medium, and the cytotoxic concentration (CC 50 ) was determined after 48 h of treatment. Additionally, the involvement of ROS was determined by measuring the cell viability in pretreated A549 cells with antioxidant NAC (1 mM) for 1 h, followed by compound 4f for 48 h.
Cell culture and cell viability assay. Human cell lines (A549 and PC-3) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured as per the guidelines supplied using the crystal violet dye uptake assay according to our previously reported method (19) (link)(20) (link)(21) (link). Cell viability assay was performed in F12K medium, and the cytotoxic concentration (CC 50 ) was determined after 48 h of treatment. Additionally, the involvement of ROS was determined by measuring the cell viability in pretreated A549 cells with antioxidant NAC (1 mM) for 1 h, followed by compound 4f for 48 h.
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