The supernatant was collected and stored at −80 °C until further analysis. The level of IL-10 and TGF-β was measured using Mouse IL-10 and TGF-β ELISA Kit (Abcam, Cambridge, UK) in accordance with the manufacturer’s protocol respectively as previously described [35 (link)].
Tgf β elisa kit
Manufactured by Abcam
Sourced in United Kingdom, China
The TGF-β ELISA kit is a quantitative assay designed to measure the concentration of transforming growth factor-beta (TGF-β) in biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify TGF-β levels.
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Lab products found in correlation
3 protocols using tgf β elisa kit
1
Quantifying IL-10 and TGF-β in Supernatant
2
Quantifying Cytokine Profiles in Cells
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Culture medium was collected for the measurement of cytokine concentration by IL-6 ELISA kit (Sino Biological, Beijing, China), IL-1β ELISA kit (Cusabio, Beijing, China), IL-10 ELISA kit (Sino biological, Beijing, China), Arginase-1 ELISA kit (Cusabio, Beijing, China), EGF ELISA kit (Abcam, Cambridge, Massachusetts), TGF-β ELISA kit (Abcam, Cambridge, Massachusetts), and HGF ELISA kit (Abcam, Cambridge, Massachusetts) according to manufacturer’s instructions. A series of cytokine concentrations (0, 5, 10, 15, 20 ng/mL) were used to plot the standard curve. The concentrations within the culture medium collected from different experimental groups were determined by applying the optical density value to the standard curve.
3
Quantify Mouse TGF-β1 in Serum
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TGF-β ELISA kit was purchased from Abcam. User manual was followed to quantify TGF-β in serum. Plates were read on SoftMax Pro Plate reader at OD of 450 nm. This kit allows for effective quantification of specifically mouse TGF-β1.
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