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Si smartpool human pex5

Manufactured by Horizon Discovery

The Si SMARTpool human PEX5 is a set of synthetic small interfering RNAs (siRNAs) designed to target the human PEX5 gene. PEX5 encodes a protein essential for the import of proteins into peroxisomes. This product is intended for research use only and its core function is to facilitate the knockdown of PEX5 expression in cell-based experiments.

Automatically generated - may contain errors

2 protocols using si smartpool human pex5

1

Peroxisome Biogenesis Knockdown in HEK 293

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On-TARGETplus ATM SiRNA (L-003201-00-0005); Si GENOME human PEX2, PEX10 and PEX12 SiRNA (M-006548-02, M-006545-00, and M-019337-02); si SMARTpool human PEX5 (M-015788-00); si SMARTpool human SQSTM1 (M-010230-00); were purchased from Dharmacon. siRNAs were resuspended in 1X siRNA buffer (GE Dharmacon) to obtain a 20 μM stock, aliquots were then frozen to prevent repeated freeze thawing. HEK 293 cells were transfected with indicated Si RNA at 10 nM final concentrations with DharmaFECT 1 or DharmaFECT 4 (GE Dharmacon) according to manufacturer's instruction, for 72 h before collection of lysates.
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2

Peroxisome Biogenesis Knockdown in HEK 293

Check if the same lab product or an alternative is used in the 5 most similar protocols
On-TARGETplus ATM SiRNA (L-003201-00-0005); Si GENOME human PEX2, PEX10 and PEX12 SiRNA (M-006548-02, M-006545-00, and M-019337-02); si SMARTpool human PEX5 (M-015788-00); si SMARTpool human SQSTM1 (M-010230-00); were purchased from Dharmacon. siRNAs were resuspended in 1X siRNA buffer (GE Dharmacon) to obtain a 20 μM stock, aliquots were then frozen to prevent repeated freeze thawing. HEK 293 cells were transfected with indicated Si RNA at 10 nM final concentrations with DharmaFECT 1 or DharmaFECT 4 (GE Dharmacon) according to manufacturer's instruction, for 72 h before collection of lysates.
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