Pdonr201

Manufactured by Transgene

PDONR201 is a lab equipment product developed by Transgene. It is a precision instrument designed for specific laboratory applications. The core function of PDONR201 is to perform a specific task within a controlled environment. No further details about its intended use or capabilities are provided.

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Lab products found in correlation

Fast mutagenesis kit, by Transgene (1 mentions) Pdonr201, by Thermo Fisher Scientific (1 mentions)

1 protocol using pdonr201

Cloning and Mutagenesis of CLE19 Promoter

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A CLE19 genomic fragment containing a 1782bp 5′ upstream region (pCLE19), 225bp coding region, and a 1205bp 3′ downstream region (tCLE19) was amplified and cloned into pDONR201 (Invitrogen) to construct the pDONR201-pCLE19:CLE19:tCLE19 entry clone. To generate a glycine to threonine substitution (G6T) at the sixth amino acid (G6) of the CLE motif, a Fast Mutagenesis Kit (TransGen, Beijing) was used to introduce point mutations into pDONR201-pCLE19:CLE19:tCLE19 to produce pDONR201-pCLE19:CLE19_G6T:tCLE19, which was then transferred to the pBGWFS7 binary vector to generate pCLE19:CLE19_G6T:tCLE19. pCLE19 and tCLE19 were cloned into the ligation-independent cloning vectors pPLV04 and pPLV15 (De Rybel et al., 2011 (link)) to generate pCLE19:SV40-3XGFP:tCLE19 and pCLE19:GUS:tCLE19, respectively. pWOX1:SV40-3XGFP, pWOX3:SV40-3XGFP, and pALE1:SV40-3XGFP were made in a similar manner using pPLV04 (De Rybel et al., 2011 (link)). The pALE1:CLE19_G6T construct was made by replacing the SV40-3XGFP cassette in pALE1:SV40-3XGFP with the CLE19_G6T coding sequence.

Xu T.T., Ren S.C., Song X.F, & Liu C.M. (2015). CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis. Journal of Experimental Botany, 66(17), 5217-5227.

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