Ethanol 95% (v/v) (Vetec, Duque de Caxias, SP, Brazil) and water filtered through a Milli-Q apparatus (Millipore, Bellerica, MA, USA) were used to prepare the extraction solutions. Methanol, acetonitrile (J. T. Baker, Center Valley, PA, USA), and Milli-Q water were used in standard, sample, and mobile phase preparations. Formic acid, acetic acid, orthophosphoric acid, trifluoroacetic acid, and trichloroacetic acid (Sigma, St. Louis, MO, USA) were used as acidifying agents for the mobile phase. Ellagic acid, gallic acid, catechin, epicatechin, rutin, hesperidin, and quercetin were of analytical grade (Sigma, St. Louis, MO, USA) and used as external standards.
Ethanol 95 v v
Manufactured by Vetec
Sourced in Brazil
Ethanol 95% (v/v) is a laboratory-grade solvent used for various applications in research and analysis. It is a clear, colorless liquid with a distinctive alcoholic odor. The concentration of ethanol in this product is 95% by volume, making it a high-purity solution suitable for use in a wide range of laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Milli q, by Merck Group (1 mentions)
Orthophosphoric acid, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Ellagic acid, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Epicatechin, by Merck Group (1 mentions)
Hesperidin, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Acetonitrile, by Tedia (1 mentions)
2 protocols using ethanol 95 v v
1
HPLC Analysis of Polyphenolic Compounds
2
Quantification of Lutein and Vitamin E
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The lutein (Achemo, Hong Kong, China) and vitamin E (Galena, São Paulo, Brazil) standards had purities of 98.80% and 90.03%, respectively. Water was obtained using the Milli-Q water purification system (Millipore, Bedford, Massachusetts, USA). The reagents acetonitrile and ethyl acetate (chromatographic grade) were purchased from Tedia (Rio de Janeiro, RJ, Brazil). Ethanol 95% (v/v) and polysorbate 80 (P80) were purchased from Vetec (Rio de Janeiro, RJ, Brazil). The samples were filtered using 10 µm polyethylene filters obtained from Hanson Research (Chatsworth, California, USA) and 0.45 µm polyvinylidene fluoride (PVDF) filters were purchased from Millex Millipore® (São Paulo, SP, Brazil).
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