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2 protocols using linezolid

1

Antimicrobial Susceptibility Testing Protocol

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Polymyxin B and ficin enzyme were obtained from Sigma-Aldrich (St. Louis, MO, USA). Polymyxin E was obtained from Kangmanlin (Nanjing, China). The following antibiotics were purchased from Yuanye Bio-technology Co., Ltd (Shanghai, China): erythromycin, lincomycin, vancomycin, ampicillin, ofloxacin, or Meilun (Dalian, China): linezolid, nisin, penicillin G, ciprofloxacin, tigecycline, and chlortetracycline.
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2

Celastrol antimicrobial activity evaluation

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All strains used in this study are listed in Table S1 (Supporting Information). Celastrol (>98% purity, HPLC; CAS No. 34157‐83‐0) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). A stock solution of celastrol (40.96 mg mL−1) was prepared in dimethyl sulfoxide (DMSO). Antibiotics namely linezolid, oxacillin, vancomycin, chloramphenicol, kanamycin, and ampicillin were purchased from Shanghai Yuanye Bio‐Technology Co., Ltd (Shanghai, China). Triton X‐100, 10% formalin, and 4% paraformaldehyde were purchased from Beijing Leagene Biotechnology Co., Ltd (Beijing, China).
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