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Rabbit anti actin

Manufactured by ABclonal
Sourced in China

Rabbit anti-actin is a primary antibody that recognizes the actin protein, which is a ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody can be used in various immunoassays, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and quantify the presence of actin in biological samples.

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2 protocols using rabbit anti actin

1

Immunoblotting Reagents and Antibodies

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Mouse control IgG (CST, 3900 S), HRP-linked horse anti-mouse IgG (CST, 7076 S) (1:2000), HRP-linked goat anti-rabbit IgG (CST,7074 S)(1:2000), Goat anti-rabbit IgG/Alexa Fluor 647 (Bioss, bs-0295G-AF647), rabbit anti-HA (CST, 3724 S)(1:1000), rabbit anti-Flag (CST, 2368 S)(1:1000), mouse anti-Flag (EASYBIO, BE2004-100), mouse anti-Myc (CST, 2276 S)(1:1000), rabbit anti-TRIM21 (proteintech, 12108-1-AP)(1:1000), rabbit anti-UBE1L (proteintech, 15818-1-AP)(1:1000), rabbit anti-UBCH8 (proteintech, 17278-1-AP)(1:1000), rabbit anti-HERC5 (GeneTex, GTX130167)(1:1000), mouse anti-HHARI (Santa Cruz biotechnology, sc-390763)(1:100), rabbit anti-ISG15 (Santa Cruz biotechnology, sc-50366)(1:500), rabbit anti-actin (ABclonal, AC004)(1:2000), rabbit anti-p62 (CST, 5114 S)(1:1000), rabbit Anti-Ubiquitin, Lys63-Specific (Merk millipore, 05-1308)(1:1000), Bafilomycin A1 (Selleck, S1413), IFNA2 (Sino Biological, 13833-HNAS), and IFN-β (Sino Biological, 10704-HNAS) were purchased from the corresponding manufactures.
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2

Comprehensive Immunoblotting Techniques

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Radioimmunoprecipitation assay (RIPA) buffer (#9806S), rabbit anti-V5 (# 13202), rabbit anti-PHB1 (#2426T), mouse anti-Flag (#8146), rabbit anti-cytochrome c (#11940T), and Alexa Fluor 488-conjugated goat anti-rabbit IgG (#4412) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-GST (#AE067), rabbit anti-actin (#AC004), rabbit anti-PHB2 (#4504), Alexa Fluor 594-conjugated goat anti-rabbit IgG (#AS039), Alexa Fluor 594-conjugated goat anti-mouse IgG (#AS054), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#AS003), HRP-conjugated goat anti-rabbit IgG (#AS014) antibodies, and FITC-phalloidin (#RM02836) were obtained from ABclonal (Wuhan, China). Rabbit polyclonal anti-GST (#bs-3725 R) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Sigma (St. Louis, MO, USA). Cellfectin® II Reagent and PLUSTM Reagent were obtained from Invitrogen (Carlsbad, CA, USA). Rhodamine 123 (#C2007) and DCFH-DA (#S0033) were obtained from Beyotime Biotechnology (Nanjing, China). Prestained protein marker (#M221) was purchased from Genstar (Beijing, China).
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