Western blot analysis was conducted using the procedure previously described (26 (link)). A luminescent image analyzer (LAS-3000, Fuji Film, Tokyo, Japan) was used to capture signals and ImageJ (NIH, Bethesda, MD, USA) was used to quantify signal levels. We used antibodies against Runx2 (8486s), Snail (3879s), TGFβ (3711s) (Cell Signaling, Danvers, MA, USA), WISP1 (AF1689, R&D_Systems, Minneapolis, MN, USA), MMP9 (sc-393859) (Santa Cruz, Dallas, TX, USA), and β-actin (A5441, Sigma, Saint Louis, MO, USA). We also employed a mouse XL cytokine array (R&D Systems, Minneapolis, MN, USA) and determined expression of 111 cytokines and chemokines in mouse urine samples.
Mmp9 sc 393859
Manufactured by Santa Cruz Biotechnology
Sourced in United States
MMP9 (sc-393859) is a lab equipment product offered by Santa Cruz Biotechnology. It is a matrix metalloproteinase-9 (MMP9) enzyme, which is responsible for the breakdown of extracellular matrix components. This product can be used for various applications in biological research.
Automatically generated - may contain errors
Lab products found in correlation
Mouse xl cytokine array, by R&D Systems (1 mentions)
Tgf β 3711 s, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Las 3000, by Fujifilm (1 mentions)
Ab175937, by Abcam (1 mentions)
Ab34710, by Abcam (1 mentions)
Gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions)
Sc 8312, by Santa Cruz Biotechnology (1 mentions)
Mmp2 sc 10736, by Santa Cruz Biotechnology (1 mentions)
Quercetin qct, by Merck Group (1 mentions)
2 protocols using mmp9 sc 393859
1
Comprehensive Protein and Cytokine Analysis
2
Quercetin Modulates Matrix Metalloproteinases
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Quercetin (QCT) was purchased from Sigma Aldrich (cat. No Q4951, St. Louis, MO, USA). Primary antibodies against MMP-28 (ab175937) and collagen I (ab34710) were obtained from Abcam (Cambridge, UK). Antibodies against total Akt kinase (sc-8312), MMP-2 (sc-10736), MMP-9 (sc-393859), TIMP-2 (sc-5539), SOD-1 (sc-17767), SOD-2 (sc-133254), and GAPDH (sc-32233) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibody recognizing phosphorylated Akt kinase (Ser473) (#4058) and secondary peroxidase-labeled anti-rabbit (#7074S) or anti-mouse (#7076S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The Colorimetric SOD Assay kit (ab65354) was purchased from Abcam (Cambridge, UK).
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