Fatty acid profiling in GhBZR3‐silenced plants was performed as previously described (Qin et al., 2007 (link)). The 5‐DPA fibers were obtained from 10 μm BZR treatment after 6 h or 12 h and immersed in chloroform/methanol (2:1, v/v) for 1 min to remove surface waxes. Then, the samples were ground to powder in liquid nitrogen and extracted using 2.5% H2SO4 (v/v) in methanol. A known quantity of heptadecanoic acid (C17:0) was added into the extraction mixture to identify percentages for the quantification of fatty acids. Then, hexane was used for the extraction of fatty acid methyl esters. The Agilent 6890 N GC system (Agilent, https://www.agilent.com) with an HP 5975 mass selective detector (Agilent) coupled to a gas chromatography mass spectrometer (GC‐MS) system was used for fatty acid detection and measurement, as described previously (Liu et al., 2015 (link)).
Hp 5975 mass selective detector
Manufactured by Agilent Technologies
The HP 5975 mass selective detector is a laboratory instrument used for the identification and quantification of chemical compounds. It functions by ionizing and fragmenting sample molecules, then analyzing the resulting ions to determine their mass-to-charge ratios. This data can be used to identify the chemical composition of the sample.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 6890n gc system, by Agilent Technologies (1 mentions)
Tlc silica gel 60 f254, by Merck Group (1 mentions)
Crizotinib, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
500 mhz spectrometer, by Bruker (1 mentions)
Hepatocyte growth factor (hgf), by Merck Group (1 mentions)
2 protocols using hp 5975 mass selective detector
1
Fatty acid profiling of GhBZR3-silenced plants
2
Synthesis and Characterization of Crizotinib
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All chemical reagents and solvents were purchased from Sigma-Aldrich or Merck suppliers and used without further purification. Crizotinib and HGF were purchased from Sigma. The progress of reactions was checked by Thin-layer chromatography (TLC) using TLC Silica gel 60 F254 (Merck, Darmstadt, Germany). A UV cabinet with wavelengths 254 and 360 nm was used for visualizing spots on the TLC plates. Column chromatography was performed using Silica gel 60 (0.040-0.063 mm), Merck, Germany. Melting points were measured in open capillaries on a Stuart Scientific apparatus and are uncorrected. IR spectra were recorded on a FT-IR Perkin Elmer spectrophotometer. NMR spectra were recorded on Bruker 500 MHz spectrometer and tetramethylsilane (TMS) was used as the standard and chemical shifts are expressed in ppm units. Coupling constants are reported in Hertz (Hz) . Low resolution mass spectra were measured using a HP 5975 Mass Selective Detector (Agilent technologies). Elemental analyses for C, H and N were performed by using a CHN elemental analyzer (GmbH-Germany), and the results are within ±0.4% of the theoretical values.
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