The concentration of CEA was determined using a Cobas e411 analyzer (Roche Diagnostics) and a ready-to-use reagent kit (Elecsys CEA; Roche Diagnostics) in individual cassettes according to the manufacturer's protocol. This detection method relies on using electrochemiluminescence to measure the immunoreactivity (20 (link)).
Elecsys cea
Manufactured by Roche
The Elecsys CEA is a laboratory equipment used for the quantitative determination of carcinoembryonic antigen (CEA) in human serum and plasma. It is an in vitro diagnostic test.
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2 protocols using elecsys cea
1
Measuring CEA Levels using Cobas e411
2
Serum Biomarkers and Immunohistochemical Analysis
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Serum NSE (neuron specific enolase), CEA (carcinoembryonic antigen), and CA19-9 (carbohydrate antigen 19–9) were measured according to the instruction of the commercial test Elecsys® NSE (Roche Diagnostics GmbH, Catalogue Number: 12133113122), Elecsys® CEA (Roche Diagnostics GmbH, Catalogue Number: 11731645322) and Elecsys® CA19-9 (Roche Diagnostics GmbH, Catalogue Number: 12133113122). The assays for each marker are designed as sandwich assay based on the Streptavidin-Biotin-technology and they were measured quantitatively on a cobas® e601 platform (Roche Diagnostics GmbH). The cut-off value for NSE (15.2 ng/mL), CEA (5 ng/mL) and CA19-9 (37 U/mL) were determined based on the reference value used in clinical practice.
Immunohistochemical analysis for tissue CgA, Synaptophysin (Syn) and CD56 was performed on a 4 μm thick paraffin-embedded tissue sections according to standardized procedure. The primary antibody used was rabbit anti-Chromogranin A monoclonal antibody (ZA-0507, ZSGB-BIO, Beijing, China), rabbit anti- Synaptophysin monoclonal antibody (ZA-0506, ZSGB-BIO, Beijing, China) and mouse anti-CD56 monoclonal antibody (ZM-0057, ZSGB-BIO, Beijing, China). The results were graded from 0 to 3 according to intensity of staining (0, negative or trace; 1, weak; 2, moderate; 3, intense). Staining of ≥ 1 was considered positive.
Immunohistochemical analysis for tissue CgA, Synaptophysin (Syn) and CD56 was performed on a 4 μm thick paraffin-embedded tissue sections according to standardized procedure. The primary antibody used was rabbit anti-Chromogranin A monoclonal antibody (ZA-0507, ZSGB-BIO, Beijing, China), rabbit anti- Synaptophysin monoclonal antibody (ZA-0506, ZSGB-BIO, Beijing, China) and mouse anti-CD56 monoclonal antibody (ZM-0057, ZSGB-BIO, Beijing, China). The results were graded from 0 to 3 according to intensity of staining (0, negative or trace; 1, weak; 2, moderate; 3, intense). Staining of ≥ 1 was considered positive.
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