Ab200807

Manufactured by Abcam

Ab200807 is a recombinant monoclonal antibody targeted against a specific antigen. It is suitable for use in various laboratory applications, such as ELISA, Western Blotting, and Immunohistochemistry. The antibody is produced in a mammalian expression system and purified using affinity chromatography.

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Lab products found in correlation

2 protocols using ab200807

Immunoblotting of Key Signaling Proteins

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The total proteins from lymphocytes were isolated by RIPA (Biorbyt, orb402072) protein lysate, separated by 10% SDS-PAGE gel electrophoresis, and transferred onto a membrane for 90 min. Then, the membrane was blocked at room temperature for 1 h using 1% BSA (Solarbio, PC0001), primary antibody dilutions MEK (Abcam, ab32091), ERK1/2 (Abcam, ab17942), p-MEK (Abcam, ab96379), p-ERK1 (Abcam, ab200807), p-ERK2 (Abcam, ab151549), PI3K (Abcam, ab106693), Akt (Abcam, ab2732), mTOR (Abcam, ab182651), p-Akt (Abcam, ab192623), p-mTOR (Abcam, ab137133), and β-actin (Abcam, ab8227) were subsequently added and incubated at room temperature for 2 h. After washing the membrane three times with TBST buffer, the cells were added with HRP-labeled secondary antibody (Abcam, ab205718) and incubated at room temperature for 0.5 h, then washed with TBST buffer for three times. The protein bands were developed by ECL luminescence kit (Absin, abs920) for detection.

Liu Y., Lu J., Dong C., Zhu L., Zhou L, & Zhu K. (2022). Hydroxyethyl Starch Improves the Prognosis of Rats with Traumatic Shock via Activation of the ERK Signaling Pathway in Lymphocytes. Computational and Mathematical Methods in Medicine, 2022, 5262189.

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Signaling Pathway Inhibitor Protocol

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Qu was purchased from Sigma-Aldrich (No. PHR1488; St Louis, MO). JSH-23, Go6983, and SCH772984 (specific inhibitors of NF-κb, PKC, and ERK1/2 respectively) and A-769662 (specific activator of AMPK) were purchased from Selleck (Houston, TX). Antibodies against uPA (ab131433), uPAR (ab82220), phosphor(p)-Pak1(ab75599), p-Limk1(ab38508), p-cofilin (ab12866), p-NF-κb (p65, S276) (ab106129), p-PKC-α (ab76016), p-PKC-β (ab75657), p-PKC-δ (ab133456), p-ERK1/2 (ab200807), and GAPDH (ab9485) that were used for western blotting were purchased by Abcam (Cambridge, England).

Li H, & Chen C. (2017). Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-κb, PKC-δ, ERK1/2, and AMPKα. Integrative Cancer Therapies, 17(2), 511-523.

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