Human pcDNA3.1/V5-His A-DKK3 construct was provided by L.Zhang (Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, Pittsburgh, PA) (60 (link)). DKK3 was silenced by lentiviral transfection with shDKK3 (Open Biosystems) and by transfection with siDKK3 (Qiagen). Stable silencing of DKK3 in HPSCs was achieved by cotransfection of lentiviral plasmid control vector or shDKK3 (HPSC-shControl or HPSC-shDKK3) with packaging vectors into 293T cells with Lipofectamine 2000 (Invitrogen). Viral supernatant (200 μl) was added to HPSCs with polybrene (8 μg/ml; hexadimethrine bromide) in a six-well plate for 2 days, and stably transduced cells were selected in puromycin (1 μg/ml). Transient silencing of DKK3 in HPSCs and PDAC cells was achieved by transfection with a mixture of 5 nM siDKK3 and 3 μl of HiPerFect agent according to the manufacturer’s recommendations (Qiagen).
Sidkk3
Manufactured by Qiagen
Sourced in United Kingdom
The SiDKK3 is a laboratory equipment product manufactured by Qiagen. It is a tool used for the detection and quantification of the DKK3 gene. The core function of the SiDKK3 is to facilitate the analysis of the DKK3 gene expression levels in various biological samples.
Automatically generated - may contain errors
2 protocols using sidkk3
1
Silencing DKK3 in Human Pancreatic Stem Cells
2
Dkk3 Silencing in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (HAC and SW1353) were transfected with 2.5 nM of siRNA
against Dkk3 (Qiagen, siDkk3) or Allstars non-targeting negative control
(Qiagen, siNegative) using Dharmafect (Thermoscientific, UK) according to
manufacturer's instructions. Cells were transfected 48 h prior to cytokine
treatment.
against Dkk3 (Qiagen, siDkk3) or Allstars non-targeting negative control
(Qiagen, siNegative) using Dharmafect (Thermoscientific, UK) according to
manufacturer's instructions. Cells were transfected 48 h prior to cytokine
treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!