Coronal or sagittal sections (35 μm thickness) were washed with 0.1 % Triton in PBS, saturated by incubation with 0.1 % Triton in PBS/5 % goat serum, and then incubated with primary antibodies as follows: AT8 anti-P-tau pSer202/Thr205 (1/500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1/1000, Abcam, #ab109390); anti-IBA1 (1/1000, Wako, #019-19741), CD68 (1/500, Bio-Rad, #MCA1957); anti-C1q (1/1000, Abcam, #ab182451). For fluorescent immunostaining, sections were incubated with the appropriate secondary antibody: anti-rabbit IgG Alexa Fluor 488 or 568 (1/2000, Invitrogen); anti-mouse IgG Alexa Fluor 488 or 568 (1/2000, Invitrogen). For non-fluorescent immunostaining, endogenous peroxidase was quenched with PBS containing 3% H2O2 for 15 min followed by amplification using the ABC system (VECTASTAIN Elite ABC HRP Kit, Vector Laboratories, Burlingame, CA, USA). Horseradish peroxidase conjugates and 3,3′-diaminobenzidine were used according to the manufacturer’s manual (Vector® DAB, Vector Laboratories, Burlingame, CA, USA). Images were obtained with an Olympus BX61 microscope and analyzed with Fiji software (ImageJ).
Anti rabbit igg alexa fluor 488 or 568
Manufactured by Thermo Fisher Scientific
Anti-rabbit IgG Alexa Fluor 488 or 568 is a fluorescent-labeled secondary antibody used to detect the presence of rabbit primary antibodies in various immunoassay techniques. The Alexa Fluor dye label provides bright and photostable fluorescence for sensitive detection.
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Lab products found in correlation
2 protocols using anti rabbit igg alexa fluor 488 or 568
1
Immunohistochemical Analysis of Tau Pathology and Neuroinflammation
2
Immunohistochemical Analysis of Tau Pathology and Neuroinflammation
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Coronal or sagittal sections (35 µm thickness) were washed with 0.1% Triton in PBS, saturated by incubation with 0.1% Triton in PBS/5% goat serum, and then incubated with primary antibodies as follows: AT8 anti-P-tau pSer202/Thr205 (1/500, Thermo Scientific, #MN1020); anti-P-tau pS396 (1/1000, Abcam, #ab109390); anti-IBA1 (1/1000, Wako, #019-19741), CD68 (1/500, Bio-Rad, #MCA1957) and anti-C1q (1/1000, Abcam, #ab182451). For fluorescent immunostaining, sections were incubated with the appropriate secondary antibody: anti-rabbit IgG Alexa Fluor 488 or 568 (1/2000, Invitrogen); anti-mouse IgG Alexa Fluor 488 or 568 (1/2000, Invitrogen). For non-fluorescent immunostaining, endogenous peroxidase was quenched with PBS containing 3% H2O2 for 15 min followed by amplification using the ABC system (VECTASTAIN Elite ABC HRP Kit, Vector Laboratories, Burlingame, CA, USA). Horseradish peroxidase conjugates and 3,3′-diaminobenzidine were used according to the manufacturer’s manual (Vector® DAB, Vector Laboratories, Burlingame, CA, USA). Images were obtained with an Olympus BX61 microscope and analyzed with Fiji software (ImageJ).
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