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Anti igf2bp3 14642 1 ap

Manufactured by Proteintech
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Sourced in China

Anti-IGF2BP3 (14642-1-AP) is a primary antibody produced by Proteintech. It is designed to detect the IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) protein. This antibody can be used in various applications such as Western Blot and Immunohistochemistry to study the expression and localization of IGF2BP3 in biological samples.

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Lab products found in correlation

Ba0412, by Boster Bio (1 mentions) Ba0315 2, by Boster Bio (1 mentions) Hrp 60004, by Proteintech (1 mentions) Ripa lysate, by Beyotime (1 mentions)

Spelling variants of this product

IGF2BP3 (13 citations) Anti-IGF2BP3 (8 citations) Anti-TM (2 citations)

2 protocols using anti igf2bp3 14642 1 ap

1

Protein Expression Analysis in Cultured Cells

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Total protein was extracted from cultured cells with a Protein Extraction Kit (Kaiji, Nanjing, China). Proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes (0.45-μM pore, Millipore). Blots were probed with anti-IGF2BP3 (14642-1-AP, Proteintech), anti-RCC2 (16755-1-AP, Proteintech), anti-BCL2 (BA0412, Boster Bio), anti-BAX (BA0315-2, Boster Bio), anti-cleaved Caspase3 (29034, Signalway Antibody), and anti-GAPDH (HRP-60004, Proteintech) antibodies. Anti-rabbit or anti-mouse HRP-conjugated secondary antibodies were used prior to ECL detection.
Zhang N., Shen Y., Li H., Chen Y., Zhang P., Lou S, & Deng J. (2022). The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability. Experimental & Molecular Medicine, 54(2), 194-205.
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2

Protein Extraction and Western Blot

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The total protein of cells was extracted with RIPA lysate (Beyotime, China). The concentration was determined by BSA standard protein and separated by SDS-PAGE. anti-IGF2BP3(14642-1-AP), anti-α-tublin (66031-1-Ig) and anti-SOX2 (66411-1-Ig) were purchased from Proteintech (Wuhan, China).
Qin S., Jin H., Li Y., Chen X., He J., Xiao J., Qin Y., Liu C., Mao Y, & Zhao L. (2024). Comprehensive analysis of IGF2BP3 with expression features, prognosis, immune modulation and stemness in hepatocellular carcinoma and pan-cancer. Journal of Cancer, 15(9), 2845-2865.
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