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Alexa fluor 546 conjugated anti mouse igg antibody

Manufactured by Thermo Fisher Scientific
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The Alexa Fluor 546-conjugated anti-mouse IgG antibody is a secondary antibody used for detection and visualization in various immunological techniques. The antibody is conjugated to the Alexa Fluor 546 fluorescent dye, which can be excited at 556 nm and emits light at 573 nm, allowing for fluorescence-based detection.

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Lab products found in correlation

Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Anti vinculin mouse monoclonal antibody, by Merck Group (1 mentions) Prolonged gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Triton x 100, by MP Biomedicals (1 mentions) Gm130, by Abcam (1 mentions) Alexa fluor 488 conjugated anti rat igg antibody, by Thermo Fisher Scientific (1 mentions) Sola15, by Thermo Fisher Scientific (1 mentions) Eclipse ni e microscope, by Nikon (1 mentions) A0262, by Agilent Technologies (1 mentions) Fluorsave reagent, by Merck Group (1 mentions) M0879, by Agilent Technologies (1 mentions) Z vad fmk, by Merck Group (1 mentions)

Close spelling variants of this product

Alexa-Fluor-546-conjugated goat anti-mouse IgG secondary antibody Alexa Fluor 546-conjugated donkey anti-mouse IgG antibody Alexa Fluor 546-conjugated anti-mouse IgG Alexa 546 conjugated-anti-mouse IgG antibody Alexa 546 conjugated anti-mouse-antibody Anti-mouse Alexa Fluor 546 Alexa Fluor 546 IgG Alexa Fluor-conjugated IgG Alexa Fluor 546 Anti-mouse Alexa Fluor

5 protocols using alexa fluor 546 conjugated anti mouse igg antibody

1

Quantitative Cell Morphology Analysis

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To assess the SKOV3ip1 and ES2 cell size, cells were cultured on dishes for 1 day. After culture, the cells were fixed in 4% paraformaldehyde in PBS (FUJIFILM Wako Pure ChemicalCorporation, Osaka, Japan) for 10 min at 37 °C and treated three times with 1% Triton X-100 (MP Biomedicals, LLC) in PBS for 10 min at room temperature. After permeabilization, the cells were stained with mouse anti-vinculin monoclonal antibody (Millipore, Billerica, MA, USA) for 2 h at 37 °C, followed by treatment with Alexa Fluor 546-conjugated anti-mouse IgG antibody (Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 488-conjugated phalloidin (Invitrogen) for 1 h at 37 °C in the dark. Prolonged gold antifade reagent with DAPI (Invitrogen) was used to mount the slides and to counterstain the cell nuclei, respectively. The observations were made using a CLSM (Olympus, Tokyo, Japan).
Ohta T., Tanaka M., Taki S., Nakagawa H, & Nagase S. (2022). Honeycomb-like Structured Film, a Novel Therapeutic Device, Suppresses Tumor Growth in an In Vivo Ovarian Cancer Model. Cancers, 15(1), 237.
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2

Visualization of Glycosyltransferase Localization

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The intracellular location of overexpressed GnTIV and GnTV was determined by immuno-fluorescence microscopy using mouse monoclonal antibodies directed against the C-terminal V5 epitope. CHO cell samples were prepared by centrifugation at 800 g for 5 min at 18°C and washed with PBS. Cells were resuspended and fixed with paraformaldehyde (6%) in PBS (1∶1). Following washing in PBS the cell suspension (20 µl) was placed on a poly-L-lysine coated slide and incubated at room temperature for 15 min. Cells were incubated with the primary V5 antibody (1∶1000) in PBS containing Tween-20 (0.01%), BSA (5%) and sodium azide (0.1%) for 1 h at room temperature and washed in PBS. The cells were then incubated with 1∶1000 dilution of a secondary goat Alexa-Fluor-546-conjugated anti-mouse-IgG antibody (Invitrogen). Slides were mounted with Vectashield containing DAPI stain (Vector Laboratories Ltd) and viewed using a Zeiss Axios fluorescent microscope. The Golgi marker antibody GM130 (Abcam) was used to locate the Golgi membrane within CHO cells.
McDonald A.G., Hayes J.M., Bezak T., Głuchowska S.A., Cosgrave E.F., Struwe W.B., Stroop C.J., Kok H., van de Laar T., Rudd P.M., Tipton K.F, & Davey G.P. (2014). Galactosyltransferase 4 is a major control point for glycan branching in N-linked glycosylation. Journal of Cell Science, 127(23), 5014-5026.
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3

Immunofluorescence Staining of Colitis

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Immunofluorescence staining was performed on five patients with untreated active colitis and five HCs. Formalin-fixed, paraffin-embedded human tissue sections 3 μm in thickness were treated in xylene, a decreasing alcohol gradient and distilled water to achieve de-waxing and rehydration of the tissue. Heat-induced epitope retrieval was performed for 15 min in Tris-EDTA buffer (pH 9). After epitope retrieval, tissue sections were permeabilized with 0.2% Triton X-100 in PBS, blocked with 5% BSA and 5% Fc receptor blocking reagant (Miltenyi Biotec) and stained with polyclonal rabbit anti-human IgA (A0262, DAKO, 1:1,000 dilution), monoclonal mouse anti-human CD79a (clone JCB117, DAKO, 1:25 dilution) and monoclonal rat anti-human Ki67 (SolA15, Invitrogen, 1:50 dilution). Staining with primary antibodies was followed by detection with polyclonal donkey Alexa Fluor 546-conjugated anti-mouse IgG antibody (Invitrogen, 1:200), Alexa Fluor 647-conjugated anti-rabbit IgG antibody (Invitrogen, 1:500) and Alexa Fluor 488-conjugated anti-rat IgG antibody (Invitrogen, 1:500). Nuclear DNA was visualized with DAPI, and coverslips were applied with FluorSave reagent (Merck Millipore). Images were acquired with either a Leica TCS SP5 upright confocal microscope or a Nikon Eclipse Ni-E microscope and were further analyzed with ImageJ software.
Uzzan M., Martin J.C., Mesin L., Livanos A.E., Castro-Dopico T., Huang R., Petralia F., Magri G., Kumar S., Zhao Q., Rosenstein A.K., Tokuyama M., Sharma K., Ungaro R., Kosoy R., Jha D., Fischer J., Singh H., Keir M.E., Ramamoorthi N., O’ Gorman W.E., Cohen B.L., Rahman A., Cossarini F., Seki A., Leyre L., Vaquero S.T., Gurunathan S., Grasset E.K., Losic B., Dubinsky M., Greenstein A.J., Gottlieb Z., Legnani P., George J., Irizar H., Stojmirovic A., Brodmerkel C., Kasarkis A., Sands B.E., Furtado G., Lira S.A., Tuong Z.K., Ko H.M., Cerutti A., Elson C.O., Clatworthy M.R., Merad M., Suárez-Fariñas M., Argmann C., Hackney J.A., Victora G.D., Randolph G.J., Kenigsberg E., Colombel J.F, & Mehandru S. (2022). Ulcerative colitis is characterized by a plasmablast-skewed humoral response associated with disease activity. Nature medicine, 28(4), 766-779.
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4

Immunohistochemical Staining of Colorectal Cancer

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Immunohistochemical staining was performed on formalin‐fixed paraffin‐embedded specimens, purchased from US Biomax (colorectal cancer tissue array, CO484a, Rockville, MD, USA). After de‐waxing in xylene and graded ethanol, the section was incubated in 3% H2O2 solution to block endogenous peroxidase activity. The section was incubated with anti‐DDX1 primary antibody (1:100) at 4°C overnight and processed using the DAB system (Vector, Burlingame, CA, USA). Paraffin sections of xenograft‐derived tumors were stained with anti‐PCNA antibody (1:100, M0879, Agilent, Santa Clara, CA, USA) in combination with AlexaFluor 546‐conjugated anti‐mouse IgG antibody (A11030, Thermo Fisher Scientific).
Tanaka K., Ikeda N., Miyashita K., Nuriya H, & Hara T. (2018). DEAD box protein DDX1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene. Cancer Science, 109(8), 2479-2489.
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5

Antibodies for Western Blot Analysis

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The Alexa Fluor 546-conjugated anti-mouse IgG antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Rabbit polyclonal anti-myosin heavy chain (MHC) antibodies (H-300, sc-20641) and anti-GAPDH antibodies (FL-335, sc-25778) , and mouse monoclonal anti-a-tubulin antibodies (B-7, sc-5286) were purchased from Santa Cruz Biotechnology. Z-VAD FMK was purchased from Sigma.
Tamura K., Takayama S., Ishii T., Mawaribuchi S., Takamatsu N, & Ito M. (2015). Apoptosis and differentiation of Xenopus tail-derived myoblasts by thyroid hormone. Journal of molecular endocrinology, 54(3).
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