The following cell lines were used: human myeloid leukemia (KG-1a, HL-60, NB-4, and HEL-R), colon cancer (Caco-2), T-lymphoblastic leukemia (Jurkat), and non-Hodgkin lymphoma (Raji). All cell lines were purchased from the American Type Culture Collection and the Leibniz-Institute DSMZ GmbH (Germany), and cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum and 1% antibiotic-antimycotic solution in a humidified chamber with 5% CO2 atmosphere at 37°C. For treatments, cells were cultured at a density of 0.2–0.4 × 106 cells/ml with a complete medium supplemented with the following inhibitors: BET inhibitor (+) JQ1 or its inactive enantiomer (-) JQ1 (100–500 nM; Cayman Chemical), BET inhibitor I-BET 762 (100–500 nM; Cayman Chemical), CDK9 inhibitor 5,6-dichlorobenzimidazole 1-β-D-ribofuranoside DRB (10–40 µM; Sigma-Aldrich), CBP/P300 inhibitor CBP30 (5–20 µM; Sigma-Aldrich), and metformin (20–60 mM; Sigma-Aldrich).
Cbp30
Manufactured by Merck Group
Sourced in United States
The CBP30 is a laboratory instrument designed for the purification and analysis of biomolecules. It utilizes column-based chromatography techniques to separate and isolate specific compounds from complex mixtures. The core function of the CBP30 is to provide high-performance liquid chromatography (HPLC) capabilities for researchers and scientists in various fields, such as biochemistry, analytical chemistry, and biopharmaceutical development.
Automatically generated - may contain errors
Lab products found in correlation
Metformin, by Merck Group (1 mentions)
Gsk126, by Cayman Chemical (1 mentions)
Human tgf β1, by Merck Group (1 mentions)
Fgf10, by Miltenyi Biotec (1 mentions)
Suberanilohydroxamic acid saha, by Merck Group (1 mentions)
Jq1 jq1, by Merck Group (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Stempro 34 sfm, by Thermo Fisher Scientific (1 mentions)
Its x, by Thermo Fisher Scientific (1 mentions)
I bet762, by Merck Group (1 mentions)
Fibroblast growth factor 2 (fgf2), by Miltenyi Biotec (1 mentions)
Sb431542, by Bio-Techne (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by Miltenyi Biotec (1 mentions)
Icbp112, by Merck Group (1 mentions)
Hexamethylene bis acetamide hmba, by Merck Group (1 mentions)
3 protocols using cbp30
1
Evaluating Epigenetic and Metabolic Modulators
2
Radiation-Induced Cell Damage Modulation
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Cells were irradiated using the137Cs Gammacell 40 Exactor (Best Theratronics, Ottawa, Ontario, Canada) at 1 Gy/min for the indicated doses. After irradiation, cells were kept in culture for the described treatment. If not otherwise indicated, cells were pretreated with (+)JQ1 (BPS Bioscience, San Diego, CA, USA), CBP30 (SigmaAldrich, St. Louis, MO, USA), GSK126 (Cat#15415, Cayman Chemical Co., Ann Arbor, MI, USA.), EPZ6438 (LKT-E6397, LKT Laboratories, Inc, St. Paul MN, USA), or vehicle (DMSO) for 48 h, irradiated with 6 Gy, and incubated for additional time with concurrent drug or vehicle treatment. Dimethyl sulfoxide (DMSO) was used to dissolve all compounds and used as vehicle control at equal volumes as for the drug treatment. Maximal DMSO concentration in assays was 0.1%.
3
Cardiomyocyte Induction from Mouse Embryonic Fibroblasts
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This protocol was modified from previous publication [31] . MEFs were seeded at a density of 10,000 cells/cm 2 and let to attach overnight. The next day, virus supernatant containing retrovirus (as described above) was added to the cells for two consecutive days. The day after, media was changed to cardiomyocytes induction media consist of StemPro-34 SFM supplemented with 1% GlutaMAX, 1 X ITS-X (Thermo Fisher), 50 μg/mL ascorbic acid (Sigma), 10 ng/mL FGF2, 50 ng/mL FGF10 and 5 ng/mL VEGF (Miltenyi Biotec). Media was changed every 3-4 days. Compounds or small molecules that were used are DMSO (Sigma), ethanol (Sigma), SB431542 (Tocris), suberanilohydroxamic acid (SAHA) (Sigma), hexamethylene bisacetamide (HMBA) (Sigma), (+)-JQ1, (-)-JQ1, I-BET 762, RVX-208, C646, CBP30, I-CBP112 and human TGF-β1 (Sigma) (all from Cayman Chemical unless specified otherwise). Stock for all small molecules were prepared in DMSO, expect for C646, CBP30 and I-CBP112, where they were prepared in ethanol, unless specified otherwise. Treatment of DMSO and all small molecules were carried out for 14 days, unless specified otherwise.
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