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Penicillin

Manufactured by Zhejiang Tianhang Biotechnology
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Penicillin is a laboratory equipment used for the production and purification of the antibiotic penicillin. It is a crucial tool in the field of biotechnology and pharmaceutical research. The core function of this equipment is to facilitate the controlled cultivation of the Penicillium fungi, the natural source of penicillin, and to extract and purify the active compound from the resulting fermentation process.

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Lab products found in correlation

Streptomycin, by Zhejiang Tianhang Biotechnology (3 mentions) Fetal bovine serum (fbs), by Zhejiang Tianhang Biotechnology (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Tianjin Damao (1 mentions) Sulfuric acid, by Tianjin Damao (1 mentions) Lyso tracker red, by Beyotime (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Hydrogen peroxide, by Tianjin Damao (1 mentions) Chlorpromazine, by Sangon (1 mentions) Rpmi 1640 culture medium, by Zhejiang Tianhang Biotechnology (1 mentions)

Close spelling variants of this product

Penicillin-streptomycin (4 citations)

4 protocols using penicillin

1

Culturing Endometrial and Placental Cancer Cells

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The human endometrial cancer cell line (Ishikawa) and placental choriocarcinoma cell line (JAR) were obtained from BeNa Culture Collection (Beijing Beina Chunglian Institute of Biotechnology) and were cultured in DMEM and RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.), respectively, supplemented with 10% FBS (cat. no. 11011-8611; Zhejiang Tianhang Biotechnology Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (cat. no. P1400; Beijing Solarbio Science & Technology Co., Ltd.) at 37°C in a humidified incubator under normoxic conditions (95% room air; 5% CO2). Cells were passaged every 3-4 days at ~80% confluence, and experiments were performed between passage numbers 3 and 5. To mimic hypoxic conditions, Ishikawa cells were maintained in a humidified incubator at 37°C with 1% O2, 5% CO2 and 94% N2 for 12, 24 or 48 h depends on the needs of the experiments. Both cell lines used in the present study were authenticated by short tandem repeat analysis.
Wang X., Miao S., Lu L., Yuan J., Pan S, & Wu X. (2022). miR-519d-3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α. International Journal of Molecular Medicine, 50(4), 123.
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2

COLO205 Cell Culture and NP Treatment

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Cell culture and treatment. Human COLO205 CRC cells were obtained from the American Type Culture Collection (ATCC CCL-222; Manassas, VA, USA). The cells were cultured in Roswell Park Memorial Institute-1640 medium (Hyclone Laboratories; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, China), 100 IU/ml penicillin and 100 mg/ml streptomycin at 37˚C in a humidified 5% CO 2 atmosphere. NP with analytical standard purity was purchased from Aladdin Industrial Corporation (Shanghai, China), and was dissolved in absolute ethyl alcohol to 50 mmol/l.
Yang X., Huang H., Wang M., Zheng X., Xu J, & Xie M. (2017). Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells. Molecular medicine reports, 16(2).
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3

Folate-Targeted Graphene Oxide Nanocarrier

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Multilayer Graphene flake was obtained from Hengqiu Tech Inc. Potassium permanganate (KMnO4, 99%), hydrochloric acid (HCl, 37%), sulfuric acid (H2SO4, 98%), hydrogen peroxide (H2O2, 30%), and anhydrous chloroform were obtained from Tianjin Damao Chemical Reagent Co. China. Doxorubicin hydrochloride (DOX) was purchased from Shanghai Yijing Industrial Co. SPC and the FA containing polyethylene glycol (PEG) distearoylphosphatidylethanolamine (DSPE-PEG2000-Folate) were bought from Ponsure Biotechnology Co. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), were purchased from Shanghai EKEAR Bio@Tech Co. LTD. LysoTracker-Red and Hoechst 33342 were obtained from Beyotime Biotechnology Co. Penicillin, streptomycin, RPMI 1640 culture medium, and fetal bovine serum (FBS) were obtained from Zhejiang Tianhang Biotechnology Co. Ltd. Double distilled water was obtained from Milli-Q system of Millipore Co., USA. Chlorpromazine (CPZ), methyl-β-cyclodextrin (MCD), and amiloride (AMI) were bought from Sangon Biotech (shanghai) Co., Ltd. Sodium dihydrogen phosphate, methanol and glacial acetic acid were purchased from Shanghai MACKLIN Chemical Reagent Co., LTD.
Ma K., Fu D., Liu Y., Rui D.a.i., Yu D., Guo Z., Cui C., Wang L., Xu J, & Mao C. (2018). Cancer cell targeting, controlled drug release and intracellular fate of biomimetic membrane-encapsulated drug-loaded nano-graphene oxide nanohybrids. Journal of materials chemistry. B, 6(31), 5080-5090.
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4

Caco-2 Cell Culture Protocol

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Caco-2 cells (Bioleaf Company, Shanghai, China) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Servicebio Technology Co., Ltd., Wuhan, China) with 25 mmol/L glucose, 4 mmol/L L-glutamine, 1 mmol/L sodium pyruvate, and phenol red, supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum (FBS) (Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, China) at 37 °C with 5% CO2.
Wei J., Zhao J., Su T., Li S., Sheng W., Feng L, & Bi Y. (2023). Flavonoid Extract from Seed Residues of Hippophae rhamnoides ssp. sinensis Protects against Alcohol-Induced Intestinal Barrier Dysfunction by Regulating the Nrf2 Pathway. Antioxidants, 12(3), 562.
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