Rabbit anti mglur5

NeutrAvidin agarose beads (catalog #29200), goat anti-rabbit (catalog #G-21234) IgG (H+L) cross-adsorbed horseradish peroxidase secondary antibody, and rabbit anti-β-actin (catalog #PA1-183) were from Thermo Fisher Scientific. Rabbit anti-mGluR 2/3 (catalog #ab6438) and anti-vinculin (catalog #ab129002) were from Abcam. Rabbit anti-mGluR5 (catalog #AB5675), anti-NMDAR2A (catalog #AB1555), anti-NMDAR2B (catalog #AB1557), anti-AMPA1 (catalog #AB1504), and anti-M1 mAChR (catalog #M9808) were from Sigma-Aldrich. Reagents used for Western blotting were from BIO-RAD, and all other biochemical reagents were from Sigma-Aldrich.

TRIzol (CAT #15596-018), Nuclease-Free Water (CAT #750023) and Power SYBR® Green PCR Master Mix (CAT # #4367659) were purchased from Thermo Fisher Scientific, Waltham, MA, USA. Vecta stain Elite ABC Kit (Mouse IgG and Rabbit IgG) and Vector SG Peroxidase Substrate Kit were purchased from Vector Laboratories (Burlingame, CA, USA). Entellan® (CAT #107960) was from Merck, Kenilworth, NJ, USA. Mouse anti-neuronal nuclei (NeuN) (Cat# MAB377, RRID: AB_2298772) and rat Horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (CAT #170-6515) were from Bio-Rad Laboratories, Hercules, CA, USA. FD Rapid GolgiStain™ (CAT #PK401) was purchased from FD NeuroTechnologies, Columbia, MD, USA. Rabbit anti-p-CREB and anti-CREB polyclonal antibody (CAT #sc-52, RRID:AB_2629503) were obtained from Cell Signaling Technology, Danvers, Massachusetts, USA. Rabbit anti-vinculin (CAT #129002) were obtained from Abcam, Cambridge, United Kingdom). ECL Western blotting detection reagents (CAT # #RPN2232) were purchased from GE Healthcare, Chicago, IL, USA. 2-chloro-4-((2,5-dimethyl-1-(4-(trifluoromethoxy) phenyl)-1H-imidazol-yl) ethynyl) pyridine (CTEP) was purchased from Axon Medchem. Rabbit anti-mGluR5 (CAT #AB5675) and all other biochemical reagents were purchased from Sigma–Aldrich, Saint Louis, MO, USA.

Sulfo-NHS-SS-biotin and Neutravidin were purchased from Thermo Scientific (Waltham, MA, USA. Mouse anti-FMRP antibody was purchased from Abcam (Cambridge, UK). DHPG was purchased from Tocris Bioscience (Bristol, UK). Myo-[³H]-inositol was purchased from Perkin Elmer (Waltham, MA, USA). Dowex I-X8 resin, rabbit anti APP antibody, rabbit anti-phospho mTOR (pSer2481) antibody and rabbit anti-mTOR antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA).Goat anti-rabbit secondary antibody was purchased from BioRad (Hercules, CA, USA). Aggregated Aβ human ELISA kit and rabbit Aβ peptide antibody were purchased from Invitrogen (Carlsbad, CA, USA). Rabbit anti-mGluR5 was purchased from Millipore (Billerica, MA, USA). Finally Vector Elite ABC kit rabbit and mouse, and Vector SG substrate was purchased from Vector laboratories. All other biochemical reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).