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Supersensitive tmb

Manufactured by Merck Group
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Supersensitive TMB is a laboratory equipment product manufactured by Merck Group. It is designed to provide highly sensitive detection capabilities for various applications. The core function of Supersensitive TMB is to enable accurate and reliable measurements, though its specific intended uses are not detailed here.

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Lab products found in correlation

Streptavidin hrp, by BD (2 mentions) T per buffer, by Thermo Fisher Scientific (2 mentions) Biotinylated rat anti igg1 clone a85 1, by BD (2 mentions) Rat anti ige clone r35 118, by BD (2 mentions) Eotaxin, by R&D Systems (2 mentions) Rantes, by R&D Systems (2 mentions)

3 protocols using supersensitive tmb

1

Quantitative Soluble LAG-3 ELISA

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Quantification of sLAG-3 was performed by in-house optimized ELISA. 96 well plates were coated with 5 μg/mL of coating antibody (anti-LAG-3 clone 11E3, Enzo Life sciences) and incubated with 100 μL of sample. A 10-point standard curve was generated using doubling dilutions of recombinant human LAG-3-Fc (Enzo Life Sciences) from 8 ng/mL to 15.6 pg/mL diluted in RPMI-1640 + 10% FBS media. sLAG-3 was detected by the addition of 0.5 μg/mL of anti-LAG-3-biotin (clone 17B4) and streptavidin HRP. Super sensitive TMB (Sigma) was used as the colorimetric substrate and after the addition of a 3% HCl stop solution, the optical density was measured at 450 nm.
Juno J.A., Stalker A.T., Waruk J.L., Oyugi J., Kimani M., Plummer F.A., Kimani J, & Fowke K.R. (2015). Elevated expression of LAG-3, but not PD-1, is associated with impaired iNKT cytokine production during chronic HIV-1 infection and treatment. Retrovirology, 12, 17.
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2

Quantification of Allergic Immune Markers

Cited 1 time
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Cytokine, chemokine and antibody concentrations were measured via ELISA. Plasma collected at the time of sacrifice was assayed for OVA-specific IgE and IgG1 similarly to previously described.30 (link) Briefly, plates were coated with 100 μg/ml OVA, and blocked with 1% BSA, prior to incubation with diluted plasma. Bound antibody was detected with biotinylated rat anti-IgG1 (clone A85–1, BD Pharmigen) or rat anti-IgE (clone R35–118, BD Pharmigen), followed by addition of streptavidin-HRP (BD Pharmigen) and supersensitive TMB (Sigma-Aldrich) as a substrate. Lung homogentates were prepared from half of the left lung as previously described27 (link) in T-PER buffer (ThermoFisher Scientific, Waltham, MA, USA). ELISAs for IL-5 (eBioscience), eotaxin (R&D Systems, Minneapolis, MN, USA), and RANTES (R&D Systems) were performed according to the manufacturers’ instructions.
Hernandez J.D., Yu M., Sibilano R., Tsai M, & Galli S.J. (2019). Development of multiple features of antigen-induced asthma pathology in a new strain of mast cell deficient BALB/c-KitW-sh/W-sh mice. Laboratory investigation; a journal of technical methods and pathology, 100(4), 516-526.
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3

Quantification of Allergic Immune Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine, chemokine and antibody concentrations were measured via ELISA. Plasma collected at the time of sacrifice was assayed for OVA-specific IgE and IgG1 similarly to previously described.30 (link) Briefly, plates were coated with 100 μg/ml OVA, and blocked with 1% BSA, prior to incubation with diluted plasma. Bound antibody was detected with biotinylated rat anti-IgG1 (clone A85–1, BD Pharmigen) or rat anti-IgE (clone R35–118, BD Pharmigen), followed by addition of streptavidin-HRP (BD Pharmigen) and supersensitive TMB (Sigma-Aldrich) as a substrate. Lung homogentates were prepared from half of the left lung as previously described27 (link) in T-PER buffer (ThermoFisher Scientific, Waltham, MA, USA). ELISAs for IL-5 (eBioscience), eotaxin (R&D Systems, Minneapolis, MN, USA), and RANTES (R&D Systems) were performed according to the manufacturers’ instructions.
Hernandez J.D., Yu M., Sibilano R., Tsai M, & Galli S.J. (2019). Development of multiple features of antigen-induced asthma pathology in a new strain of mast cell deficient BALB/c-KitW-sh/W-sh mice. Laboratory investigation; a journal of technical methods and pathology, 100(4), 516-526.
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