Phosphate buffered saline (PBS. 0.01 M phosphate buffer, 0.0027 M potassium chloride, 0.137 M sodium chloride) was used for analysis. PBS tablets (P4417) were purchased from Sigma-Aldrich, UK. The control study proteins used were γ-globulin from human blood (G4386), albumin from human serum (A9511) and fibrinogen from human plasma (F3879), all sourced from Sigma Aldrich, UK. Streptavidin surface modified SPBs were purchased from Ademtech, France (03121), with a known mean diameter of 125 nm and a measured concentration of 1 × 10 12 mL -1 and used in all experiments. Invitrogen dynabeads were used, mean size of 1.05 μm.
Fibrinogen from human plasma f3879
Manufactured by Merck Group
Sourced in United Kingdom
Fibrinogen from human plasma (F3879) is a laboratory product provided by Merck Group. It is a naturally occurring protein that plays a crucial role in the blood clotting process. This product can be used for various research and analytical purposes, but a detailed description of its intended use is not provided to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Albumin from human serum a9511, by Merck Group (2 mentions)
Dynabead, by Thermo Fisher Scientific (1 mentions)
Pbs tablets p4417, by Merck Group (1 mentions)
Arium 611 uv water purification system, by Sartorius (1 mentions)
Nih3t3 fibroblast cell lines atcc crl 1658, by American Type Culture Collection (1 mentions)
Triethoxysilane tes, by Tokyo Chemical Industry (1 mentions)
2 protocols using fibrinogen from human plasma f3879
1
Phosphate Buffered Saline for Biomolecular Analysis
2
Protein-Enhanced Silane Functionalization
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Triethoxysilane (TES) was purchased from Tokyo Chemical Industry and used as received. 1-Decene and proteins were purchased from Sigma Aldrich (St.Louis, MO, USA). Albumin from human serum (A9511), fibrinogen from human plasma (F3879), and transferrin human (T3309) were purchased from Sigma-Aldrich. All other chemicals were purchased from Wako Pure Chemical Industries (Tokyo, Japan) and used as received. Water was purified and deionized using a Sartorius (arium 611 UV) water purification system (Sartorius AG, Goettingen, Germany). HeLa (RCB0007) and A549 (RCB0098) cell lines were obtained from the Riken Bio-Resource Center (Tsukuba, Japan). NIH3T3 fibroblast cell lines (ATCC® CRL-1658) were obtained from ATCC (ATCC, Manassas, VA, USA).
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