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Facs cytexpert 2

Manufactured by Beckman Coulter
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Sourced in United States

The FACS CytExpert 2.0 is a flow cytometry system designed for cell analysis and sorting. It provides advanced data acquisition and analysis capabilities to support various research applications.

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Lab products found in correlation

Annexin 5 fitc pi staining kit, by Keygen Biotech (1 mentions)

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CytExpert 2.0 software (237 citations) CytExpert (205 citations)

2 protocols using facs cytexpert 2

1

Cellular ROS Measurement by DCFH-DA

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Cellular ROS production was detected using a 2’,7’-dichlorofluorescein diacetate (DCFH-DA) probe24 (Beyotime, China) according to the manufacturer’s protocol. Briefly, a total of 1.5 × 105/ml cells in 1 ml was seeded in 12-well dishes. The treated cells were washed with PBS twice and incubated with 10 μM of DCFH-DA for 30 min. DCFH-DA fluorescence was measured by a FACS CytExpert 2.0 (Beckman Coulter, USA).
Wang Y., Wang C.Q., Sun P., Liu P.P., Yang H., Wang H.Y., Rao H.L., Li S., Jiang W.Q., Huang J.J, & Li Z.M. (2022). Phase II Study of Gemcitabine, Peg-Asparaginase, Dexamethasone and Methotrexate Regimen for Newly Diagnosed Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type: Final Analysis With Long-Term Follow-Up and Rational Research for the Combination. Frontiers in Oncology, 12, 796738.
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2

Apoptosis Detection via Annexin V-FITC/PI

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Apoptosis was then detected with annexin V-FITC/PI staining kit (Nanjing KeyGen Biotech). NKYS, SNK6 cells were treated with normal conditions, MTX (20 mM), gemcitabine (5 μM), or combination treatment for 24-48 hr, respectively. The cells were then collected, washed, and stained in a working solution (500 µl binding buffer with 5 µl annexin V-FITC and 10 µl PI) for 15 min at room temperature in the dark. The FACS CytExpert 2.0 (Beckman Coulter) was used to detect and analyze apoptotic cells. Annexin V-FITC + and annexin V-FITC +-PI + cells were considered as apoptotic cells.
Wang Y., Wang C.Q., Sun P., Liu P.P., Yang H., Wang H.Y., Rao H.L., Li S., Jiang W.Q., Huang J.J, & Li Z.M. (2022). Phase II Study of Gemcitabine, Peg-Asparaginase, Dexamethasone and Methotrexate Regimen for Newly Diagnosed Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type: Final Analysis With Long-Term Follow-Up and Rational Research for the Combination. Frontiers in Oncology, 12, 796738.
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