D xylulose

Manufactured by Merck Group

Sourced in Sao Tome and Principe

D-xylulose is a monosaccharide used in biochemical research and laboratory applications. It is a five-carbon sugar that can be utilized as a substrate or intermediate in various metabolic processes and enzymatic assays.

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3 protocols using d xylulose

Enzymatic Assays of Carbohydrates

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d-erythrose, l-erythrulose, d-erythritol, d-arabitol, l-arabinose, d-xylose, d-ribose, and d-xylulose were obtained from Sigma-Aldrich (St. Louis, United States). Coomassie brilliant blue R-250, cofactor NADP, NAD, NADH, NADPH, and antibiotics were purchased from Sangon Biotech (Shanghai, China). The chromatographic media were procured from GE Healthcare Life Sciences (Sweden). All other chemicals were of analytical grade and used as such without any purification.

Cheng H., Wang S., Bilal M., Ge X., Zhang C., Fickers P, & Cheng H. (2018). Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering. Microbial Cell Factories, 17, 133.

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Furfural Production from d-Xylose

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d-Xylose (>98%) from Alfa Aesar was used as a raw material for furfural production. d-Xylose (>99%), d-xylulose (>98%), d-lyxose (99%) and furfural (>98.5%) obtained from Sigma-Aldrich were used for product analysis. Aluminum chloride (>95%) and formic acid (88%) were purchased from Alfa Aesar. Acetonitrile (99.9%) used as a mobile phase for the analysis of product was obtained from RCI Labscan. Amberlyst 21 was purchased from Alfa Aesar for the acid catalyst removal process.

Tongtummachat T., Jaree A, & Akkarawatkhoosith N. (2022). Continuous hydrothermal furfural production from xylose in a microreactor with dual-acid catalysts. RSC Advances, 12(36), 23366-23378.

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Synthesis of Nucleotide Sugars

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D-ribose, D-ribulose, and D-ribitol were purchased from Carbosynth. D-galactose was from Merck Millipore. Cytidine triphosphate, D-xylulose, D-xylose, L-arabinose, doxycycline, and 1 mol/L triethylammonium acetate were from Sigma. CMP-N-acetylneuraminic acid, UDPgalactose, UDP-glucose, UDP-N-acetylgalactosamine, UDP-N-acetylglucosamine, GDP-mannose, GDP-fucose, ADP-ribose, UDP-xylose, and UDP-glucuronic acid were from Sigma or Carbosynth.
Rbo5P was produced as previously described (5 ) . CDP-ribitol was synthesized in vitro from Rbo5P and Cytidine triphosphate with recombinantly produced human CRPPA enzyme from Escherichia coli (kind gift of Dr. W. Yue, Oxford) (5 ) . The internal standard, a 13 Cyeast extract containing [U- 13 C]-UDP-glucose, was a kind gift of Dr. G. Hermann, Vienna.

van Tol W., van Scherpenzeel M., Alsady M., Riemersma M., Hermans E., Kragt E., Tasca G., Kamsteeg E.J., Pennings M., van Beusekom E., Vermeulen J.R., van Bokhoven H., Voermans N.C., Willemsen M.A., Ashikov A, & Lefeber D.J. (2019). Cytidine Diphosphate-Ribitol Analysis for Diagnostics and Treatment Monitoring of Cytidine Diphosphate-l-Ribitol Pyrophosphorylase A Muscular Dystrophy. Clinical chemistry, 65(10).

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