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2 protocols using ethyl octanoate

1

Volatile Compounds Analysis in Wine

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Ultrapure 18.3 MΩ⋅cm water used for these experiments was produced from a Zeneer Power III TOC system (Human Corp., Seoul, Korea). All the following reagents were purchased from Sigma Aldrich, unless stated otherwise, and were standard grade: butyl acetate, hexanal (Alfa Aesar, Haverhill, MA, USA), butanol, 3-methyl-1-butanol (Alfa Aesar), (E)-hex-2-enal (Alfa Aesar), hexanol, (Z)-hex-3-en-1-ol (Alfa Aesar), nonanal, 2-ethyl-1-hexanol, acetophenone, α-terpineol, benzyl alcohol, 2-phenylethanol, ethyl tetradecanoate, ethyl dodecanoate, methyl 2-hydroxybenzoate (Alfa Aesar), citronellol, ethyl decanoate, benzaldehyde (Alfa Aesar), ethyl octanoate, ethyl heptanoate, isoamyl acetate, limonene, 2-methyl-1-butanol, ethyl hexanoate, ethyl acetate, ethyl butanoate, Saccharomyces cerevisiae (Franke, Italy), (NH4)2HPO4 (Franke, Italy), K2S2O5 (Franke, Italy), tartaric acid, polyvinylpolypyrrolidone (Alfa Aesar), sodium azide (Alfa Aesar), NaOH, Na2PO4(H2O)2, citric acid, HCl, 3-octanol, MeOH, CH2Cl2, β-glucosidase enzyme (Ecozim AROM, CRC Biotek, Roma, Italy), hydrocarbon mixture from C8–C23 (Sigma-Aldrich, St. Louis, MO, USA).
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2

Quantification of phenolics and volatiles in food

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Commercial standards of delphinidin 3-O-sambubioside (DS), cyanidin 3-O-sambubioside (CS) and quercetin, were purchased from Extrasynthese, Genay, (France), while trifluoroacetic acid, LC-MS grade formic acid, gallic acid, caffeic acid, Folin-Ciocalteu's reagent and ethanol were from Sigma Aldrich, St Louis, Missouri, (USA). 2-phenylethanol, isoamyl alcohol, ethyl acetate, ethyl hexanoate, ethyl decanoate, ethyl lactate, isoamyl acetate, methyl butyrate, intestinal acetone powder from rat, glucose assay reagent, maltose and sodium mono and dibasic phosphate were from Sigma-Aldrich, Dorset, (UK); while 1hexanol, eugenol, diethyl succinate and ethyl octanoate were from Alfa Aesar, Lancashire, (UK). Sodium carbonate was from Fisher scientific, Loughborough (UK) and the solid phase extraction Oasis MAX cartridge 3 mL (60 mg) was purchased from Waters Corporation Ltd., Massachusetts (USA). All other standards used in the study were either analytical grade, HPLC or GC grade.
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