Medical grade pcl

MEW scaffolds were fabricated by a previously described custom-built MEW [37] . Medicalgrade PCL (Corbion Inc, Netherlands, PURASORB PC12, Lot# 1412000249, 03/2015) was used to fabricate the MEW scaffolds. All MEW printing was performed at 21 ± 2 °C environment temperature and a humidity of 35 ± 10%. PCL was heated at 80 °C in 3 mL syringe and air pressure was set to 3 bar. A high voltage was applied and after an electrified jet was generated, the G-code that drive the collector was initiated. A large 48 x 96 mm rectangular mesh with 100 µm, 200 µm or 400 µm spacing fiber was direct-written and then cut with an infrared laser to 9 mm circular disks for use in 24-well plates for in vitro experiments. MEW scaffolds are produced using the following parameters: 25 G nozzle and a 6 kV voltage applied across a 4 mm collector distance.