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Cellometer cytometer

Manufactured by Revvity
Sourced in United States

The Cellometer cytometer is a compact and automated cell counting and analysis instrument. It utilizes advanced optical technology to accurately measure cell concentration and viability in a variety of samples.

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2 protocols using cellometer cytometer

1

Apoptosis Measurement by Flow Cytometry

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After treatment with either DMSO or MRBE (20 and 40 μg/ml) and ursolic acid (20 and 40 μM) for 48 h, cells were washed with cold phosphate-buffered saline and stained with annexin V-FITC and PI. The levels of apoptosis were then measured using the Apoptosis Detection Kit (BD Transduction Laboratories), according to the manufacturer’s protocols. Cells were then analyzed using a Cellometer cytometer (Nexcelom, USA).
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2

Isolation of Murine Spleen and Lymph Nodes

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Spleen and lymph nodes were removed from mice aseptically from naive 6–8 week old mice and kept on media on ice. Single cell suspensions were obtained by rubbing the tissue between two frosted glass slides and suspending on ice in RPMI-1640 (Gibco-Life/Thermo Scientific, Waltham, MA) + 10% FBS (Genesee Scientific, San Diego, CA). Cell counts were determined by counting with AO/PI stained cells in a Cellometer cytometer (Nexcelom Bioscience, Lawrence, MA).
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