The human retinal pigment epithelial cell line (ARPE-19 cells) was from the Fu Heng Cell Center (Shanghai, China). It was cultured with 10% FBS, penicillin (50 U/mL), and streptomycin (50 U/mL at 37°C in a humidified atmosphere with 5% CO2. Next, CeO2 NPs were dispersed in ultrapure water to prepare stock solutions (200 mg/mL). The stock solution was sonicated using a probe sonicator (Ningbo Xinzhi Biotechnology Co., Ltd., China) at 600 W for 40 min and diluted to different concentrations with culture medium and penicillin/streptomycin just before cell exposure. The cells were adjusted to a concentration of 1×105 cells/mL in a volume of 100 μL per well in 96-well plates for toxicity assays.
Probe sonicator
Manufactured by Ningbo Xinzhi
Sourced in China
The Probe Sonicator is a laboratory instrument used for the homogenization and dispersion of samples through the application of ultrasonic energy. It is designed to disrupt the cellular structure of samples, facilitating the extraction or separation of specific components.
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Lab products found in correlation
2 protocols using probe sonicator
1
Cytotoxicity Assay of CeO2 Nanoparticles
2
Preparation and Characterization of Celastrol-Loaded Nanoparticles
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CEL-NPs were prepared using an emulsion/solvent evaporation method. Briefly, PEG2000-PLGA20000 and CEL were dissolved in chloroform to form the oil phase. The resultant oil phase was then added into an aqueous phase. The mixture was emulsified by sonication with a probe sonicator (Ningbo Xinzhi Biotechnology Co. Ltd.; Ningbo, China). CEL-NPs were then prepared after the chloroform was evaporated at low pressure. CEL-RNPs were prepared as indicated above using PEG2000-PLGA20000, MAL-PEG2000-PLGA20000, and RGD-PEG2000-PLGA20000, and CEL was dissolved in chloroform to form the oil phase. To obtain CEL-PRNPs, mPEG2000-GPLGLAGQC was conjugated with CEL-RNPs in PBS with pH 7.4 at room temperature for 4 h52 (link). Unconjugated mPEG2000-GPLGLAGQC was removed by elution through a Sephadex G-75 column.
The particle sizes and zeta potentials of CEL-NPs, CEL-RNPs, and CEL-PRNPs were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZS90 instrument (Malvern Panalytical, Malvern, UK). The encapsulation efficiency (EE%) was determined by ultrafiltration method. The morphology of CEL-NPs, CEL-RNPs, and CEL-PRNPs was determined by TEM (H-600, Hitachi, Japan).
To study the serum stability, the prepared CEL-NPs, CEL-RNPs, and CEL-PRNPs were stored in 10% FBS at 37 °C for 24 h. Changes in particle size were determined by DLS.
The particle sizes and zeta potentials of CEL-NPs, CEL-RNPs, and CEL-PRNPs were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZS90 instrument (Malvern Panalytical, Malvern, UK). The encapsulation efficiency (EE%) was determined by ultrafiltration method. The morphology of CEL-NPs, CEL-RNPs, and CEL-PRNPs was determined by TEM (H-600, Hitachi, Japan).
To study the serum stability, the prepared CEL-NPs, CEL-RNPs, and CEL-PRNPs were stored in 10% FBS at 37 °C for 24 h. Changes in particle size were determined by DLS.
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