Rabbit anti tsg101

The following antibodies were used in this study: mouse monoclonal anti-TBEV E (United States Army Medical Research, Institute of Infectious Diseases, Fort Detrick, Frederick, MD, USA), mouse monoclonal anti-TBEV prM (United States Army Medical Research), mouse monoclonal anti-TSG101 (Santa Cruz), mouse J2 anti-dsRNA (SCICONS, Hungary), mouse monoclonal anti-ALIX (Santa Cruz), mouse monoclonal anti-CHMP4A (Santa Cruz), rabbit anti-TSG101 (Atlas antibodies), rabbit anti-CHMP4A (Invitrogen), rabbit anti-ALIX (Atlas antibodies), rabbit anti-HA tag (Invitrogen), mouse anti-HA tag (Invitrogen), rapid anti-GRP94 (Invitrogen), mouse anti-GAPDH (Sigma), mouse anti-βactin (Sigma), mouse monoclonal anti-mCherry tag (Novus Biological), rabbit monoclonal anti-mCherry tag (Novus Biological), Alexa Fluor 594-conjugated anti-mouse goat antibody (Invitrogen), Alexa Fluor 488-conjugated anti-rabbit goat antibody (Invitrogen), anti-mouse VisUCyte HRP Polymer (R&D Systems, USA), and HPR-conjugated anti-mouse goat antibody (Invitrogen).

Primary antibodies used were rabbit anti‐p65 from Santa Cruz (clone C‐20, ref. sc‐372) at 1/250, mouse anti‐myc9E10 (developed by Bishop, J.M. was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at the University of Iowa), mouse anti‐HA (Eurogentec, clone 16B12, ref. MMS‐101R), rabbit anti‐HA (Sigma, ref. H6908), rabbit anti‐GFP (Amsbio, ref. TP401), rabbit anti‐UBAP1 (Proteintech, ref. 12385‐1‐AP), mouse anti‐His (Sigma, clone HIS‐1, ref. H1029), mouse anti‐FLAG (Sigma, clone M2, ref. F1804) all at 1/1000 and mouse anti‐TNFR1 (Santa Cruz, clone H‐5, ref. sc‐8436) and rabbit anti‐TSG101 (Atlas Antibodies, ref. HPA006161) both at 1/200. Rabbit polyclonal anti‐PumA serum was obtained by repeated immunization of rabbits with purified PumA (Eurogentec) and was used at 1/1,000 for Western blot and for immunofluorescence microscopy. Purified BtpA was used to obtain chicken anti‐BtpA (Eurogentec). Anti‐EF‐Tu antibody (kind gift from R. Voulhoux) was used at 1/10,000.
Secondary antibodies used were anti‐rabbit, mouse, chicken or rat conjugated with Alexas‐488, ‐555 or ‐647 all from Jackson ImmunoResearch. When necessary, phalloidin‐568 (1/1,000) was used to label the actin cytoskeleton and DAPI nuclear dye (1/1,000) for the host cell nucleus. For Western blots, anti‐mouse or rabbit‐HRP antibodies were used at 1/5,000.