Edu imaging kit

Manufactured by Keygen Biotech

Sourced in China

The EdU Imaging kit is a laboratory tool designed for the detection and visualization of cells undergoing DNA synthesis. It provides a method to label and identify proliferating cells in a sample. The kit includes necessary reagents and components to perform this analysis.

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Lab products found in correlation

Fluorescence microscope, by Olympus (2 mentions) Hoechst 33342, by Beyotime (1 mentions) Hoechst, by Keygen Biotech (1 mentions)

3 protocols using edu imaging kit

EdU Imaging of bMSC Proliferation

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EdU Imaging kit (Nanjing KeyGen Biotech Co., Ltd.) was used, according to the manufacturer’s protocols to detect TMP roles in bMSC proliferation. A total of 200 μl bMSCs at P₇ were seeded in 24-well plates (7 × 10³ cells/well). After 6 h of adherence culture, bMSCs were exposed to TMP treatment as stated above. A total of 10 μl 5-ethynyl-2′-deoxyuridine (EdU) (20 μM) was added and incubated for 24 h at 37°C with 5% CO₂. The cells were fixed with 4% polyformaldehyde for 15 min at room temperature. kFluor488 azide reagent and Hoechst33342 (0.5 μg/ml; Beyotime) were used to stain the nuclei for 15 min at room temperature. The images were captured under a fluorescence microscope.

Song X., Dai J., Li H., Li Y., Hao W., Zhang Y., Zhang Y., Su L, & Wei H. (2019). Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling. Bioscience Reports, 39(6), BSR20190761.

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Cell Proliferation Assay via EdU Imaging

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EdU Imaging Kit (KeyGEN, Nanjing, China) was used for evaluating cell proliferation according based on the manufacturer's instruction. 5 μg/mL Hoechst (KeyGEN) was used for the staining of cell nuclei. EdU-positive cell number was measured by fluorescence microscope (Olympus, Tokyo, Japan).

Wu T., Qi W., Shan H., Tu B., Jiang S., Lu Y, & Wang F. (2021). Ginsenoside Rg1 enhances the healing of injured tendon in achilles tendinitis through the activation of IGF1R signaling mediated by oestrogen receptor. Journal of Ginseng Research, 46(4), 526-535.

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Cell Proliferation Assay by EDU-DAPI

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The proliferation ability was detected by EDU Imaging Kit (KeyGen) in the treated H460 and A549 cells. In accordance with the manufacturer's instruction, cells were labeled with EDU and cell nuclei were stained with diamidine phenylindole (DAPI). The stained cells were observed under the fluorescence microscope (Olympus). The merged cells by EDU and DAPI staining were regarded as the positive proliferation cells.

Zhu J., Wang F., Weng Y, & Zhao J. (2022). Exosome‐delivered circSATB2 targets the miR‐330‐5p/PEAK1 axis to regulate proliferation, migration and invasion of lung cancer cells. Thoracic Cancer, 13(21), 3007-3017.

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