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Prb 155p

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The PRB-155P is a versatile and reliable laboratory device designed for precise and consistent measurements. It features a durable construction and accurate performance to meet the demands of various research and testing applications. The core function of the PRB-155P is to provide accurate and reliable measurements, ensuring consistent and reliable data for your laboratory needs.

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Lab products found in correlation

Prb 160p, by Fortrea (5 mentions) Prb 159p, by Fortrea (3 mentions) Hpa034881, by Merck Group (2 mentions) Phospho histone h3, by Cell Signaling Technology (2 mentions) Ab24647, by Abcam (2 mentions) Ab170933, by Abcam (2 mentions) Cm405 a, by Biocare Medical (2 mentions) Anti β catenin, by Cell Signaling Technology (2 mentions) Mab 9 1 56, by Abcam (2 mentions) Prb 14p, by Abcam (2 mentions) Anti ki67, by Abcam (2 mentions) Spm269, by Fortrea (2 mentions) Anti c myc, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Lsm 880, by Zeiss (2 mentions) Western blocking reagent, by Roche (1 mentions) Troma 1, by Fortrea (1 mentions) Af3625, by R&D Systems (1 mentions) Ab15580, by Abcam (1 mentions) Mab424, by Merck Group (1 mentions)

16 protocols using prb 155p

1

Immunofluorescence Analysis of Cytoskeleton

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A fraction of each replicate from the FACSVantage SE Turbo mock sort experiment (unsorted and mock sorted) was fixed in 4% paraformaldehyde (PBS). Cells were permeabilized in 0.5% Triton X-100. Cells were blocked/stained in 1x western blocking reagent (Roche, 11921673001). Cells were stained with rat a-cytokeratin 8 (1:250) (DSHB; (U of Iowa), TROMA-1) and rabbit a-cytokeratin 14 (1:500) (Covance, PRB-155P). Cells were mounted and then imaged using a Zeiss 710-LSM. A total of 25 fields of view were recorded (per-replicate/condition) and then counted for CK81 and CK141 cells.
Richardson G.M., Lannigan J, & Macara I.G. (2015). Does FACS perturb gene expression?. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 87(2).
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2

Immunostaining Protocol for Epithelial Markers

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Mouse monoclonal antibody against IL-33 (clone Nessy-1) (#ALX-804-840-C100) was purchased from Enzo. Rabbit polyclonal antibodies against KRT5 (#ab24647) and Ki-67 (#ab15580) were purchased from Abcam (Abcam, Cambridge, MA). Rabbit polyclonal antibody against KRT14 (#PRB-155P) was purchased from Covance (Covance, Princeton, NJ). Rabbit polyclonal antibody against KRT4 (#HPA034881) was purchased from Sigma (Sigma-Aldrich Corp, St. Louis, MO). Rabbit monoclonal antibodies against E-cadherin (#3195), p75 (#8238), and phospho-histone H3 (#3377) were purchased from Cell Signaling (Cell Signaling Technology, MA). Mouse monoclonal antibody against p63 (#sc-8431) was purchased from Santa Cruz (Santa Cruz Biotechnology, TX). Mouse monoclonal antibody against PCNA (#MAB424) was purchased from Millipore (Billerica, MA). Goat polyclonal antibody against IL-33 (#AF3625) was purchased from R&D (R&D Systems, Minneapolis, MN). Donkey anti-goat Alexa Fluor 488 (A11055), anti-rabbit Alexa Fluor 568 (A10042), and anti-mouse Alexa Fluor 647 (A31571) secondary antibodies were purchased from Life Technologies (Carlsbad, CA). Mouse anti-HSP90 (TA500494) and mouse anti-GAPDH (TA310153) primary antibodies were purchased from Origene (Rockville, MD).
Travers J., Rochman M., Caldwell J.M., Besse J.A., Miracle C.E, & Rothenberg M.E. (2017). IL-33 is induced in undifferentiated, non-dividing esophageal epithelial cells in eosinophilic esophagitis. Scientific Reports, 7, 17563.
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3

Immunohistochemical Profiling of Breast Cancer

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The detailed methods and results of the IHC analysis of expression of: KRT5/6 (PRB-160P, Covance, 1/2000; 0.5 µg/mL), KRT14 (PRB-155P, Covance, 1/2000; 0.5 µg/mL), GATA3 (CM405 A, Biocare Medical, 1/300) and FOXA1 (ab170933, Abcam, 1/100; 10 µg/mL) in this patient series have been reported elsewhere (13 (link)). Tumors were classified in three subtypes according to the IHC results: BASQ-like (FOXA1/GATA3 low; KRT5/6/14 high), luminal-like (FOXA1/GATA3 high; KRT5/6/14 low), and mixed (FOXA1/GATA3 high; KRT5/6 high; KRT14 low).
Font A., Domenech M., Ramirez J.L., Marqués M., Benítez R., Ruiz de Porras V., Gago J.L., Carrato C., Sant F., Lopez H., Castellano D., Malats N., Calle M.L, & Real F.X. (2023). Predictive signature of response to neoadjuvant chemotherapy in muscle-invasive bladder cancer integrating mRNA expression, taxonomic subtypes, and clinicopathological features. Frontiers in Oncology, 13, 1155244.
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4

Immunofluorescence Profiling of Esophageal Biopsies

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Immunofluorescence staining was performed as previously described 4 (link) on at least four distal esophageal biopsies from control individuals, distal esophageal biopsies with active inflammation from patients with active EoE, or proximal esophageal biopsies without active inflammation from the same patients with active EoE. The average ages of EoE patients and control individuals were 13 and 11 years, respectively. Patient groups were also controlled for race and sex. The following primary antibodies were used at a final concentration of 1 μg/mL: anti–keratin 14 (PRB-155P, Covance), anti–keratin 5 (ab24647, Abcam), anti–keratin 4 (HPA034881, Sigma), or anti-cornulin (AF3607, R&D). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Slides were blocked with phosphate-buffered saline with 10% goat or donkey serum. Secondary antibodies (1:500 dilution) used were donkey anti-rabbit Alexa Fluor 647 (A31573, Life Technologies), goat anti-rabbit Alexa Fluor 647 (A21244, Life Technologies), or donkey anti-goat Alexa Fluor 488 (A11055, Life Technologies). Imaging was performed with a Nikon A1 inverted confocal microscope.
Rochman M., Travers J., Miracle C.E., Bedard M.C., Wen T., Azouz N.P., Caldwell J.M., KC K., Sherrill J.D., Davis B.P., Rymer J.K., Kaufman K.M., Aronow B.J, & Rothenberg M.E. (2017). Profound Loss of Esophageal Tissue Differentiation in Eosinophilic Esophagitis. The Journal of allergy and clinical immunology, 140(3), 738-749.e3.
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5

Immunohistochemical Analysis of Bladder Cancer

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TURBT formalin-fixed paraffin-embedded (FFPE) blocks were reviewed. Representative tumor areas were selected to extract 3 cores and construct tissue microarrays (TMAs). Cores were positioned non-consecutively to avoid artefacts; TMAs were constructed following the established guidelines and sectioned; and the slides were embedded in paraffin and stored at 4 °C. The following antibodies were used: KRT5/6 (PRB-160P, Covance; 1/2000), KRT14 (PRB-155P, Covance, 1/2000), GATA3 (CM405 A, Biocare Medical, 1/300), FOXA1 (ab170933, Abcam, 1/100), FGFR3 (B9, Santa Cruz), KRT20 (Ks20.8, Dako), and STAG2 (J-12, Santa Cruz). After deparaffinization, antigen retrieval, and endogenous peroxidase blockade, the sections were incubated with antibodies overnight, washed, and Envision secondary reagents (Agilent) were added for 1 h. After washing, the reactions were developed with DAB, the sections were counterstained with hematoxylin, and mounted. Scoring was performed blind to clinical/pathological information by an experienced investigator (FXR). The proportion of reactive cells (0–100%) and staining intensity (0–3) were recorded; the histoscore was calculated as the product: Intensity x % reactive cells (range 0–300). The average of the scores was used for analysis.
Font A., Domènech M., Benítez R., Rava M., Marqués M., Ramírez J.L., Pineda S., Domínguez-Rodríguez S., Gago J.L., Badal J., Carrato C., López H., Quer A., Castellano D., Malats N, & Real F.X. (2020). Immunohistochemistry-Based Taxonomical Classification of Bladder Cancer Predicts Response to Neoadjuvant Chemotherapy. Cancers, 12(7), 1784.
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6

Comprehensive Immunohistochemical Profiling

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For histology, tissues were fixed in the 10% formalin, blocked in paraffin, sectioned as 5 µm thick, stained with hematoxylin and eosin, and examined by light microscopy. Antibodies against ERalpha (sc-542; Santa Cruz), PR (sc-166169; Santa Cruz), ERBB2 (sc-33684; Santa Cruz), and MRC2 (sc-271148; Santa Cruz) were used for immunohistochemical staining, by using a Histostain® Plus Broad Spectrum kit (859043; Life Technologies) as per the manufacturer's instruction. Antibodies against Keratin14 (PRB-155P; Covance), Keratin18 (sc-53256; Santa Cruz), E-Cadherin (3195; Cell Signaling Technology) and Vimentin (5741; Cell Signaling Technology) were used for immunoflouresencent staining, and nuclei were stained with DAPI (62248; ThermoFisher Scientific).
Sun H., Zeng J., Miao Z., Lei K.C., Huang C., Hu L., Su S.M., Chan U.I., Miao K., Zhang X., Zhang A., Guo S., Chen S., Meng Y., Deng M., Hao W., Lei H., Lin Y., Yang Z., Tang D., Wong K.H., Zhang X.D., Xu X, & Deng C.X. (2021). Dissecting the heterogeneity and tumorigenesis of BRCA1 deficient mammary tumors via single cell RNA sequencing. Theranostics, 11(20), 9967-9987.
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7

Multiparametric Immune Cell Profiling

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Primary antibodies used include anti-NK1.1 (1:200; 553162; BD Biosciences), anti-F4/80 (1:200; 123122; BioLegend), anti-neutrophil-elastase (1:200; ab68672; Abcam), anti-K14 (1:200; PRB-155P; Covance), anti-CD49b (1:200; StemCell Technologies), anti-CD3 (1:200; StemCell Technologies), anti-CD44 (1:200; 14-0441-82; eBioscience), anti-IFN-γ (1:100; 11-7311-82; Thermo Fisher Scientific), and DAPI (1:1,000; D571; Invitrogen). All secondary antibodies used were Alexa Fluor conjugates (1:200; Invitrogen). Treatment of monocultures and cocultures described in the main text were performed with anti-TIGIT antibody (clone 1G9, BE0274; BioXCell) and anti-KLRG1 antibody (clone 2F1, 16-5893-82; Thermo Fisher Scientific).
Chan I.S., Knútsdóttir H., Ramakrishnan G., Padmanaban V., Warrier M., Ramirez J.C., Dunworth M., Zhang H., Jaffee E.M., Bader J.S, & Ewald A.J. (2020). Cancer cells educate natural killer cells to a metastasis-promoting cell state. The Journal of Cell Biology, 219(9), e202001134.
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8

Immunofluorescence and Immunoblotting Antibodies

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For immunofluorescence microscopy, the following primary antibodies were used: monoclonal anti-T7 (Covance; 9115), anti-SRSF6 (CSHL; mAb 9 1-56), anti-keratin 6 (Abcam; SPM269) and polyclonal anti-keratin 5 (Covance; PRB-160P), anti-keratin 14 (Covance; PRB-155P), anti-loricrin (Covance; PRB-14P), anti-KI67 (Epitomics; 15580), anti-c-myc (Abcam; 39688), anti-β-catenin (Cell Signaling; 9587). For immunoblotting, the antibodies used were: monoclonal anti-SRSF6 (CSHL; mAb 9 1-56), anti-GAPDH (Abcam; 6C5), anti-TNC (4C8MS; Novus Biologicals; 4C8MS) and polyclonal anti-keratin 5 (Covance; PRB-160P). For tissue microarray analysis, monoclonal anti-SRSF6 (CSHL; mAb 9 1-56) was used. Typical antibody dilutions were 1: 25 for IHC and 1:1000 for immunoblotting.
Jensen M.A., Wilkinson J.E, & Krainer A.R. (2014). Splicing factor SRSF6 promotes hyperplasia of sensitized skin. Nature structural & molecular biology, 21(2), 189-197.
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9

Frozen Tissue Immunohistochemistry Protocol

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Frozen sections were processed and antibodies used as described (25 (link), 35 (link)). Other antibodies include GFP (Sigma G1544) and keratin-14 (Covance PRB155P). Nuclei were stained using Sytox Green (Life Technologies) or bisbenzamide. Images were acquired using Nikon C1si Laser Scanning Confocal Microscope.
Unger G.M., Kren B.T., Korman V.L., Kimbrough T.G., Vogel R.I., Ondrey F.G., Trembley J.H, & Ahmed K. (2014). Mechanism and efficacy of sub-50 nm tenfibgen nanocapsules for cancer cell-directed delivery of anti-CK2 RNAi to primary and metastatic squamous cell carcinoma. Molecular cancer therapeutics, 13(8), 2018-2029.
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10

Immunohistochemical Analysis of Keratin 14 and COX2 Expression

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Immunohistochemical staining was performed using keratin 14 polyclonal antibody (Covance, PRB-155P; 1:1,000). Every sample was scored for its CK14 staining by determining the percentage of visual fields that were CK14 negative (0%), CK14 low-infiltrating (<25%), CK14 high-infiltrating (25–100%), and CK14 basal-restricted (staining restricted to the basal layer of urothelium). The final result for each case was the average score of all visual fields. COX2 staining was performed on serial sections using COX2 monoclonal antibody (Cayman Chemical, 160112; 1:100).
Kurtova A.V., Xiao J., Mo Q., Pazhanisamy S., Krasnow R., Lerner S.P., Chen F., Roh T.T., Lay E., Ho P.L, & Chan K.S. (2014). Blocking PGE2-induced tumour repopulation abrogates bladder cancer chemoresistance. Nature, 517(7533), 209-213.
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