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Sw1417

Manufactured by American Type Culture Collection
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Sourced in United States

The SW1417 is a laboratory centrifuge designed for general purpose applications. It is capable of reaching a maximum speed of 17,000 rpm and can accommodate a variety of rotor types to suit different sample volumes and tube sizes. The SW1417 provides reliable and consistent performance for a range of centrifugation needs in research and clinical laboratory settings.

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Lab products found in correlation

Hct116, by American Type Culture Collection (10 mentions) Sw480, by American Type Culture Collection (6 mentions) Colo205, by American Type Culture Collection (5 mentions) Hct15, by American Type Culture Collection (4 mentions) Sw620, by American Type Culture Collection (4 mentions) Colo678, by Leibniz Institute DSMZ (4 mentions) Colo320, by American Type Culture Collection (3 mentions) Caco 2, by American Type Culture Collection (3 mentions) Sw480, by Thermo Fisher Scientific (3 mentions) Ls411n, by American Type Culture Collection (2 mentions) Mycoalert mycoplasma detection kit, by Lonza (2 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Mda mb 468, by American Type Culture Collection (2 mentions) Bt474, by American Type Culture Collection (2 mentions) Skbr3, by American Type Culture Collection (2 mentions) Sk mel 2, by American Type Culture Collection (2 mentions) Mda mb 157, by American Type Culture Collection (2 mentions) Hcc1937, by American Type Culture Collection (2 mentions) Mia paca 2, by American Type Culture Collection (2 mentions)

15 protocols using sw1417

1

Colorectal Cancer Cell Lines

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The human CRC cell lines SW480, RKO, and SW1417 were purchased from the ATCC. COLO320 and LOVO were purchased from RIKEN. LIM1215 was purchased from the ECACC. HT‐29 was provided by Dr Mariadason at the Ludwig Institute for Cancer Research (Heidelberg, Australia). SW480 was cultured in RPMI‐1640 (Sigma‐Aldrich) with 10% FBS, whereas the other six cell lines were grown in DMEM (Sigma‐Aldrich) with 10% FBS.
Hiraide S., Takahashi M., Yoshida Y., Yamada H., Komine K, & Ishioka C. (2021). Tumor suppressor miR‐193a‐3p enhances efficacy of BRAF/MEK inhibitors in BRAF‐mutated colorectal cancer. Cancer Science, 112(9), 3856-3870.
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2

Cell Line Cultivation for Cancer Research

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Cell lines were purchased from ATCC or provided by R.B.: (1) BRAFV600E CRC cells: WiDr, SNUC5, HT29, Colo-205, RKO-1, LIM2405, KM20, LS411N, VACO432, SW1417; (2) CRC MAP3K8amp cells: OUMS23; (3) KRASmut CRC cells: HCT116, LoVo; (4) BRAFV600E melanoma cells: A375, A375 (SRCY530F), A375 (myr-AKT1), Sk-Mel-28, Mel888. Cells were cultured following ATCC’s instructions or as previously described13 (link).
Ruiz-Saenz A., Atreya C.E., Wang C., Pan B., Dreyer C.A., Brunen D., Prahallad A., Muñoz D.P., Ramms D.J., Burghi V., Spassov D.S., Fewings E., Hwang Y.C., Cowdrey C., Moelders C., Schwarzer C., Wolf D.M., Hann B., VandenBerg S.R., Shokat K., Moasser M.M., Bernards R., Gutkind J.S., van ‘t Veer L.J, & Coppé J.P. (2023). A reversible SRC-relayed COX2 inflammatory program drives resistance to BRAF and EGFR inhibition in BRAFV600E colorectal tumors. Nature Cancer, 4(2), 240-256.
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3

Characterization of Human Colon Cancer Cell Lines

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Human colon cancer cell lines CACO2 (catalog no. HTB-37, RRID:CVCL_0025), DLD-1 (catalog no. CCL-221, RRID:CVCL_0248), HCT15 (catalog no. CCL-225, RRID:CVCL_0292), HCT116 (catalog no. CCL-247, RRID:CVCL_0291), HT29 (catalog no. HTB-38, RRID:CVCL_0320), LOVO (catalog no. CCL-229, RRID:CVCL_0399), LS411N (catalog no. CRL-2159, RRID:CVCL_1385), RKO (catalog no. CRL-2577, RRID:CVCL_0504), SW480 (catalog no. CCL-228, RRID:CVCL_0546), SW1417 (catalog no. CCL-238, RRID:CVCL_1717), and mouse macrophages RAW 264.7 (catalog no. TIB-71, RRID:CVCL_0493) were purchased from the ATCC. KM12 (RRID:CVCL_1331), OUMS23 (RRID:CVCL_3088), and SW48 (RRID:CVCL_1724) human colon cancer cell lines were kind gifts from W. Hahn. These cell lines were authenticated using short tandem repeat profiling (Labcorp). KM12 cells did contain extra peaks next to the main markers exhibited by MSI-high cell lines. CACO2, KM12, OUMS23, RKO, SW480, and SW1417 cells were cultured in DMEM media. DLD-1, HCT15, LS411N, and LOVO cells were cultured in RPMI media. HCT116 and HT29 cells were cultured in McCoy's 5A media. Human embryonic kidney 293T (catalog no. CRL-3216, RRID:CVCL_0063) cells were cultured in DMEM media. All media were supplemented with 10% FBS and 2 mmol/L glutamine (Gibco, catalog no. 25030081) and cells were cultured at 37°C under 5% CO2. Cell lines were tested to confirm lack of Mycoplasma contamination.
Miller A.L., Perurena N., Gardner A., Hinoue T., Loi P., Laird P.W, & Cichowski K. (2023). DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon Cancer. Cancer Research, 83(11), 1800-1814.
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4

Cell Line Validation for Research

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HEK293 and HCT116 cell lines were obtained from ATCC, and have been maintained in the laboratory for several years. All other hCCCs used in the current studies, including COLO-205, RKO, COLO-320, and SW1417 cell lines were purchased from ATCC in 2015 and confirmed by ATCC. CCD841 cells were purchased from ATCC in 2015 by Dr. Carla Kantara (UTMB, Galveston, TX) and confirmed by ATCC and were kindly gifted to us. All cell lines were monitored regularly for absence of mycoplasma, and HEK293 and HCT116 cell lines were confirmed to represent human epithelial cell lines with the help of Biosynthesis, Inc., as described previously (12 (link)).
Sarkar S., O’Connell M.R., Okugawa Y., Lee B.S., Toiyama Y., Kusunoki M., Daboval R.D., Goel A, & Singh P. (2017). FOXD3 Regulates CSC Marker, DCLK1-S, and Invasive Potential: Prognostic Implications in Colon Cancer. Molecular cancer research : MCR, 15(12), 1678-1691.
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5

Colorectal Cancer Cell Culture

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SW620, HCT116, C2BB, and SW1417 were purchased from the American Type Culture Collection. The cells were cultured in modified McCoys’ 5A medium (Gibco, 16600082) supplemented with 10% FBS (ATCC, 30-2020) and 1% penicillin-streptomycin (ATCC, 30-2300). They were incubated under a 5% (v/v) CO2 atmosphere at 37 °C.
Bian Y., Alem D., Beato F., Hogenson T.L., Yang X., Jiang K., Cai J., Ma W.W., Fernandez-Zapico M., Tan A.C., Lawrence N.J., Fleming J.B., Yuan Y, & Xie H. (2022). Development of SOS1 inhibitor-based degraders to target KRAS-mutant colorectal cancer. Journal of medicinal chemistry, 65(24), 16432-16450.
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6

Sourcing and Characterizing Colorectal Cancer Cell Lines

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HCT116, SW48, WiDr, SW1417, SNU-C2B, SNU-2CA, and SW480 were sourced from the American Type Culture Collection (ATCC). LIM2099 was purchased from MilliporeSigma (Burlington, MA, USA). SW837 was sourced from Amgen (Thousand Oaks, CA, USA), DiFi was donated from Robert Coffey (Vanderbilt University Medical Center Nashville, TN, USA), RW7213 was donated from Sanjay Goel’s lab (Montefiore Medical Park, NY, USA) and SNU-1411 was donated from Sandra Misale’s lab (Sloan Kettering Institute, NY, USA). All cell lines had biweekly tests for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit from Lonza Walkersville, Inc. (LT07-418 Walkersville, MD, USA). Cell lines were cultured using RPMI 1640 supplemented with 10% FBS and 1% penicillin and streptomycin from Gibco (Waltham, MA, USA).
Davis T.B., Gupta S., Yang M., Pflieger L., Rajan M., Wang H., Thota R., Yeatman T.J, & Pledger W.J. (2022). Ras Pathway Activation and MEKi Resistance Scores Predict the Efficiency of MEKi and SRCi Combination to Induce Apoptosis in Colorectal Cancer. Cancers, 14(6), 1451.
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7

Culturing Human Colon Cancer Cell Lines

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Human colon cancer cell lines Caco-2, SW403, SW1417, COLO 205, HT-29, HCT 116, and RKO were supplied by the American Type Culture Collection (ATCC, Rockville, MD, USA); the HT115 cell line was obtained from Sigma-Aldrich, Milan, Italy. All cell lines were cultured following the manufacturer’s instructions; culture media were supplemented with fetal bovine serum (HyClone, Cramlington, UK), 100 U/mL penicillin, 100 mg/mL streptomycin, and 250 ng/mL Amphotericin-B. The identity of all cell lines was confirmed by short tandem repeat (STR) profiling; cells were routinely screened for mycoplasma contamination with MycoAlert mycoplasma detection kit (Lonza, Milan, Italy).
Sellitto A., Geles K., D’Agostino Y., Conte M., Alexandrova E., Rocco D., Nassa G., Giurato G., Tarallo R., Weisz A, & Rizzo F. (2019). Molecular and Functional Characterization of the Somatic PIWIL1/piRNA Pathway in Colorectal Cancer Cells. Cells, 8(11), 1390.
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8

Cell Line Culture and Maintenance

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RKO, WiDr, MCF7, T47D, MDA-MB-468, SW1417, NCI-H358, MIAPaCa-2, NCI-H1573, NCI-H1792, SKMEL2, Calu-1, Calu-6, HCT15, SW620, HCT116, HCC1937, BT20, MDA-MB-231, MDA-MB-157, Hs 578T, HT-29, SK-BR-3, BT-474, T84, and LoVo cells were purchased from the American Type Culture Collection (ATCC). HeLa, MDA-MB-436, BT-549, and SUM159 were provided by Ramon Parsons, and SNU387 cells were provided by Amaia Lujambio (both at Icahn School of Medicine at Mount Sinai). SW1736, Hth104, and 8505C cells were provided by James Fagin (Memorial Sloan Kettering Cancer Center). Cell lines were maintained in a humidified incubator at 37° C with 5% CO2, cultured in RPMI 1640, DMEM, DMEM/F12, or F12 supplemented with 10% FBS, 2 mM glutamine and 100 IU/ml penicillin and streptomycin.
Ahmed T.A., Adamopoulos C., Karoulia Z., Wu X., Sachidanandam R., Aaronson S.A, & Poulikakos P.I. (2019). SHP2 Drives Adaptive Resistance to ERK Signaling Inhibition in Molecularly Defined Subsets of ERK-Dependent Tumors. Cell reports, 26(1), 65-78.e5.
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9

Cell Line Culture and Maintenance

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RKO, WiDr, MCF7, T47D, MDA-MB-468, SW1417, NCI-H358, MIAPaCa-2, NCI-H1573, NCI-H1792, SKMEL2, Calu-1, Calu-6, HCT15, SW620, HCT116, HCC1937, BT20, MDA-MB-231, MDA-MB-157, Hs 578T, HT-29, SK-BR-3, BT-474, T84, and LoVo cells were purchased from the American Type Culture Collection (ATCC). HeLa, MDA-MB-436, BT-549, and SUM159 were provided by Ramon Parsons, and SNU387 cells were provided by Amaia Lujambio (both at Icahn School of Medicine at Mount Sinai). SW1736, Hth104, and 8505C cells were provided by James Fagin (Memorial Sloan Kettering Cancer Center). Cell lines were maintained in a humidified incubator at 37° C with 5% CO2, cultured in RPMI 1640, DMEM, DMEM/F12, or F12 supplemented with 10% FBS, 2 mM glutamine and 100 IU/ml penicillin and streptomycin.
Ahmed T.A., Adamopoulos C., Karoulia Z., Wu X., Sachidanandam R., Aaronson S.A, & Poulikakos P.I. (2019). SHP2 Drives Adaptive Resistance to ERK Signaling Inhibition in Molecularly Defined Subsets of ERK-Dependent Tumors. Cell reports, 26(1), 65-78.e5.
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10

Authentication of BRAF Mutant Colorectal Cell Lines

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The BRAFV600E colorectal cancer cell lines were sourced as follows; COLO 201, COLO 205, HT29, SW1417 and RKO (American Type Culture Collection, Manassas, VA, USA), LIM2551 and LIM2405 cells (Ludwig Institute for Cancer Research), CO115 [22 (link)], VACO432 [23 (link)], LS411 [24 (link)] and VACO5 [25 (link)]. All cell lines were maintained in Dulbecco’s Minimal Essential Media/F12 (DMEM/F12, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 5% FBS (v/v)(Moregate, Queensland, Australia) at 37 °C with 5% CO2. Cell line authentication was performed by short tandem repeat (STR) profiling using the GenePrint 10 system (Promega, Madison, WI, USA), and all lines were found to be exact matches to published profiles. Mycoplasma testing was performed every 3-6 months as part of routine monitoring in our laboratory.
Jenkins L.J., Luk I.Y., Chionh F., Tan T., Needham K., Ayton J., Reehorst C.M., Vukelic N., Sieber O.M., Mouradov D., Gibbs P., Williams D.S., Tebbutt N.C., Desai J., Hollande F., Dhillon A.S., Lee E.F., Merino D., Fairlie W.D, & Mariadason J.M. (2024). BCL-XL inhibitors enhance the apoptotic efficacy of BRAF inhibitors in BRAFV600E colorectal cancer. Cell Death & Disease, 15(3), 183.
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